转基因玉米CM8101实时荧光定量PCR检测方法的建立

doi:10.3969/j.issn.1674-7968.2021.05.017

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Abstract Genetically modified maize (Zea mays) CM8101 is one of the transgenic maize lines with commercial prospect developed independently in China. The research of quantitative detection method for CM8101 transgenic maize and its products is one of the technical requirements for the safety supervision of transgenic crops. In this study, real-time fluorescence quantitative primers and TaqMan probe were designed according to the 5' side sequence information of transgenic maize CM8101. After specificity, linearity, limit of detection, limit of quantification, accuracy, precision and reproducibility tests, a specific real-time fluorescence PCR quantitative detection method for CM8101 maize was established.The results of sample determination showed that this quantitative method had strong specificity and good accuracy, and could accurately quantify CM8101 transgenic components as low as 0.1%, and the detection limit could reach to 10 copies. In this study, an accurate quantitative detection method for CM8101 transgenic maize was developed based on qRT-PCR technology, which is expected to provide necessary technical support for CM8101 transgenic corn safety supervision.

Key words： Genetically modified maize CM8101 Specific detection Quantitative realtime PCR Quantitative detection

Received: 02 October 2020 Published: 01 May 2021

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Corresponding Authors: *lifeiwu3394@sina.com

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	Articles by authors
	LONG Li-Kun
	ZHAO Ning
	LI Cong-Cong
	HE Yu-Xuan
	DONG Li-Ming
	YAN Wei
	LI Fei-Wu

Cite this article:

LONG Li-Kun,ZHAO Ning,LI Cong-Cong, et al. Establishment of qRT-PCR Method of Detection Transgenic Maize (Zea mays) CM8101[J]. 农业生物技术学报, 2021, 29(5): 1007-1015.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.05.017 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I5/1007

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