扭果花旗杆<em>DeTIP2;1</em>基因的克隆、亚细胞定位和表达分析

doi:10.3969/j.issn.1674-7968.2021.12.002

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Abstract AQPs (aquaporins) play an important role in plant development and abiotic stress resistance. In this study, DeTIP2;1, the only up-regulated gene of AQPs family, was screened from drought transcriptome data of Xinjiang xerophyte Dontostemon elegans. Cloning the full-length sequence, bioinformatics analysis, expression analysis and subcellular localization were carried out. ：The results showed that the total length of CDS was 750 bp, encoding 249 amino acids, with a molecular mass of 24.92 kD and a theoretical isoelectric point of 5.30, and contained 2 highly conserved domain of asparagine-proline-alanine (NPA) motifs.. Both drought and salt stress could affect the expression of DeTIP2;1, and the expression was tissue specific. Subcellular localization showed that DeTIP2;1 was located in tonoplast. The above results showed that DeTIP2;1 may be involved in water absorption and transportation in the root of D. elegans. This study provides data supports for the mining and screening of functional genes of xerophytic resources in arid areas.

Key words： Dontostemon elegans D. elegans tonoplast intrinsic protein (DeTIP2;1) Bioinformatics Subcellular localization Expression analysis

Received: 24 April 2021

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Corresponding Authors: *mastergfw@163.com

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	ZOU Li-Yuan
	MA Wei
	LI Jin-Yu
	GE Feng-Wei

Cite this article:

ZOU Li-Yuan,MA Wei,LI Jin-Yu, et al. Cloning, Subcellular Localization and Expression Analysis of DeTIP2;1 Gene from Dontostemon elegans[J]. 农业生物技术学报, 2021, 29(12): 2279-2288.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.12.002 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I12/2279

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