|
|
|
| Identification of Mango (Mangifera indica) DBB Genes and Its Expression Analysis During the Infection of Colletotrichum gloeosporioides and Xanthomonas campestris pv. mangiferaeindicae |
| SUN Yu1,2, LIU Zhi-Xin2, YE Zi1,2, GAO Ai-Ping3, LUO Rui-Xiong3, PU Jin-Ji1,2, ZHANG He1,2,* |
1 Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education/College of Plant Protection, Hainan University, Haikou 570228, China;
2 Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs/Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
3 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China |
|
|
|
|
Abstract B-box protein is a kind of zinc finger structure transcription factor, its function is universal and specific. In order to reveal the sequence and expression characteristics of the double B-box (DBB) gene family in Mangifera indica, in this study, based on the whole genome data of mango, using bioinformatics methods to analyze the sequence of mango DBB gene family, and the relative expression level of DBB gene family was studied by qRT-PCR during the infection of Colletotrichum gloeosporioides (Cg) and Xanthomonas campestris pv. mangiferaeindicae (Xcm). Nine DBB gene family members were identified from the mango genome and named MiDBB1~9. The 9 MiDBBs proteins were unstable hydrophilic acidic proteins, mainly located in the nucleus. Not only was the tertiary structures similar, but also the N-terminal were 2 conserved domains (B-box1 and B-box2) with the same site. According to protein structure prediction, phylogenetic relationship, conservative motifs and multiple sequence comparison analysis, it was inferred that the DBB gene family of mango was evolutionarily conserved in structure and similar in function. The phylogenetic tree constructed by mango with Arabidopsis thaliana, Nicotiana tabacum, Malus domestica, Ananas comosus and Populus trichocarpa showed that the DBB of mango were closely related to those of the P. trichocarpa, followed by apple. The results of qRT-PCR showed that during the infection of C. gloeosporioides, the relative expression of MiDBB1~9 continued to increase within 12~48 h. During the infection of X. campestris pv. mangiferaeindicae, the relative expression levels of MiDBB2 and MiDBB3 continued to increase within 12~72 h. It was preliminarily speculated that the expression of DBB gene in mangosteen was induced by C. gloeosporioides and X. campestris pv. mangiferaeindicae. The above findings provide reference for the follow-up study of the functions and mechanisms of mango DBB gene family members.
|
|
Received: 29 April 2021
|
|
|
|
Corresponding Authors:
*atzzhef@163.com
|
|
|
|
[1] 刘焱, 邢立静, 李俊华, 等. 2012. 水稻含有B-box锌指结构域的OsBBX25蛋白参与植物对非生物胁迫的响应[J]. 植物学报, 47(4): 366-378.
(Liu Y, Xing L J, Li J H, et al.2012. Rice B-box zinc finger protein OsBBX25 is involved in the abiotic response[J]. Bulletin of Botany, 47(4): 366-378.)
[2] 娄永峰, 杨丽, 彭镇华, 等. 2015. 毛竹4个B-box锌指蛋白序列和基因表达特征及PeBZF4的功能[J]. 林业科学, 51(03): 34-40.
(Lou Y F, Yang L, Peng Z H, et al.2015. Protein sequences and expression profiles of 4 B-box zinc finger genes and functions of PeBZF4 from Phyllostachys edulis[J]. Scientia Silvae Sinicae , 51(03): 34-40.)
[3] 蒲金基, 张贺, 周文忠. 2015. 芒果病害综合防治技术[J]. 中国热带农业, 03: 38-42.
(Pu Jin-ji, Zhang He, Zhou Wen-zhong.2015. Integrated control technology of mango diseases[J]. China Tropical Agriculture, 03: 38-42.)
[4] 汪启明, 屠小菊, 赵小英, 等. 2009. 受光调节的拟南芥锌指蛋白DBB (Double B-box)亚家族基因的转录表达[J]. 植物生理学通讯, 45(8): 785-790.
(Wang Q M, Tu X J, Zhao X Y, et al.2009. Transcription of zinc finger protein DBB (Double B-Box) subfamily respond to light in Arabidopsis thaliana[J]. Plant Physiology Communications, 45(8): 785-790.)
[5] 王日红, 宋敏燕, 王然, 等. 2019. 山梨B-box基因PuBBX24表达特性及其在童期调控中的功能分析[J]. 园艺学报, 46(08): 1458-1472.
(Wang R H, Song M Y, Wang R, et al.2019. Expression Pattern and Function in Juvenile Regulation of B-box Gene PuBBX24 in Pyrus ussuriensis[J]. Acta Horticulturae Sinica, 46(08): 1458-1472.)
[6] 孙宇, 刘志鑫, 叶子, 等. 2021. 杧果Whirly基因鉴定及其在病菌侵染过程中的表达分析[J]. 西北植物学报, 41(01): 37-45.
(Sun Y, Liu Z X, Ye Z, et al.2021. Identification of mango whirly gene and its expression analysis in the pathogen infection[J]. Acta Botanica Boreali-Occidentalia Sinica, 41(01): 37-45.)
[7] 叶一隽, 李佳敏, 曹红利, 等. 2021. 茶树CsBBX基因家族的鉴定与表达[J]. 应用与环境生物学报, 27(3): 1-15.
(Ye Y J, Li J M, Cao H L, et al.2021. Identification and expression analysis of the CsBBX gene family in tea plants[J]. Chinese Journal of Applied & Environmental Biology, 27(3): 1-15.)
[8] 喻群芳, 漆艳香, 张辉强, 等. 2019. 4种生防菌对芒果细菌性黑斑病的田间防效[J]. 中国热带农业, 03: 23-25.
(Yu Q F, Qi Y X, Zhang H Q, et al.2019. Field control effect of four kinds of biocontrol bacteria on mango bacterial black spot disease[J]. China Tropical Agriculture, 03: 23-25.)
[9] 张贺, 韦运谢, 漆艳香, 等. 2015. 温湿度对芒果炭疽病病原菌分生孢子萌发及附着胞形成的影响[J].中国植保导刊, 35(01): 10-13.
(Zhang H, Wei Y X, Qi Y X, et al.2015. Effects of temperature and humidity on conidium germination and appressorium formation of Colletotrichum gloeosporioides[J]. China Plant Protection, 35(01): 10-13.)
[10] Bailey T L, Boden M, Buske F A, et al.2009. MEME SUITE: Tools for motif discovery and searching[J]. Nucleic Acids Research, 37(2): 202-208.
[11] Finn R D, Coggill P, Eberhardt R Y, et al.2016. The Pfam protein families database: Towards a more sustainable future[J]. Nucleic Acids Research, 44(D1): D279-D285.
[12] Gasteiger E, Gattikr A, Hoogland C, et al.2003. ExPASy: The proteomics server for in-depth protein knowledge and analysis[J]. Nucleic Acids Research, 31(13): 3784-3788.
[13] Kim Y S, An C, Park S, et al.2017. CAMTA-mediated regulation of salicylic acid immunity pathway genes in Arabidopsis exposed to low temperature and pathogen infection[J]. The Plant Cell, 29(10): 2465-2477.
[14] Klug A, Schwabe J W.1995. Protein motifs 5. Zinc fingers[J]. The FASEB Journal: Official publication of the Federation of American Societies for Experimental Biology, 9(8): 597-604.
[15] Kumar S, Stecher G, Tamur K.2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 33(7): 1870-1874.
[16] Laity J H, Lee B M, Wright P E.2001. Zinc finger proteins: New insights into structuraland functional diversity[J]. Current Opinion in Structural Biology, 11(14): 39-46.
[17] Livak K J, Schmittgen T D.2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method[J]. Methods-A Companion To Methods in Enzymology, 25(4): 402-408.
[18] Luo C, He X H, Chen H, et al.2013. Molecular cloning and expression analysis of four actin genes (MiACT) from mango[J]. Biobogia Plantarum, 57(2): 238-244.
[19] Mackay J P, Crossley M.1998. Zinc fingers are sticking together[J]. Trends Biochem Science, 23(1): 1-4.
[20] Ohama N, Sato H, Shinozaki K, et al.2017. Transcriptional regulatory network of plant heat stress response[J]. Trends in Plant Science, 22(1): 53-65.
[21] Prasad K V S K, Abdrl-Hameed A A E, Xing D, et al.2016. Global gene expression analysis using RNA-seq uncovered a new role for SR1 / CAMTA3 transcription factor in salt stress[J]. Scientific Reports, 6(1): 443-448.
[22] Riechmann J L, Heard J, Martin G, et al.2000. Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes[J]. Science, 290(12): 2105-2110.
[23] Schwechheimer C, Bevan M.1998. The regulation of transcription factor activity in plants[J]. Trend in Plant Science, 3(10): 278-283.
[24] Sanchez-Garcia I, Rabbitts T H.1994. The LIM domain: A new structural motif foundin zinc-finger-like proteins[J]. Trends in Genetics, 10(9): 315-320.
[25] Torok M, Etkin L D.2001. Two B or not two B? Overview of the rapidly expanding B-box family of proteins[J]. Differentiation, 67(3): 67-71.
[26] Wei H, Wang P, Chen J, et al.2020. Genome-wide identification and analysis of B-BOX gene family in grapevine reveal its potential functions in berry development[J]. BMC Plant Biology, 20(1): 72-90.
[27] Wang P, Luo Y F, Huang J F, et al.2020. The genome evolution and domestication of tropical fruit mango[J]. Genome Biology, 21(1): 60-76. |
|
|
|
