Cloning and Expression Analysis of Prophenoloxidase (PPO2) Gene of the Gomphocerus sibiricus
ADILA A-Ji, LIAO Jie-Jie, MA Ai-Xia, WEI Xiao-Jia, YE Xiao-Fang, JI Rong, HU Hong-Xia*
Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang/International Center for the Collaborative Management of Cross-border Pest in Central Asia/College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China
Abstract Phenoloxidases (POs) are important immune effector molecules of insect and belong to the type 3 copper protein familywith oxidoreductase activities. Generally, PO is present as a zymogen, prophenoloxidase (PPO), which is activated by a serine protease cascade upon recognition of foreign invaders, and then catalyzes both melanin biosynthesis and the oxidation of phenols to quinones. Gomphocerus sibiricus is dominant pest in alpine and subalpine grassland areas in Xinjiang. The study of immune related gene of G. sibiricus can lay a foundation for the future study of its interaction with pathogenic organisms to carry out biological control. The full length cDNA of PPO2 were cloned by RACE technology in this study, and which consisted of 2 439 bp (GenBank No. KY981766). Bioinformatic analysis indicated that PPO2 is highly similar to Locusta migratoria and Diabolocatantops pinguis. It contained 2 common copper-binding regions, 2 potential proteolytic activation sites, a plausible thiolester site, and a conserved C-terminal region. Genetic expression analysis showed that the gene expression level was the highest inhindgut of G. sibiricus. The expression level in posterior intestine was significantly higher than midgut, and there were no difference between salivary glands and malpighian tubules, the expression was least in fat body. This study could provide a theoretical basis for further study of the function of PPO2 gene in G. sibiricus.
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