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| Population Genetic Structure and Pathogenicity Analysis of Cochlibolus heterostrophus from Sweet Corn (Zea mays) in Fujian Province |
| DAI Yu-Li1, GAN Lin1, LIAO Lei1, TENG Zhen-Yong2, LU Xue-Song1, YANG Xiu-Juan1,* |
1 Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2 Fujian Seed Station, Fuzhou 350001, China |
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Abstract Southern corn leaf blight (SCLB) caused by Cochlibolus heterostrophus is an important foliar fungal disease that significantly affects corn (Zea mays) yield and quality. To determine the population genetic structure and pathogenicity of C. heterostrophus populations from sweet corn in different geographical locations, inter-simple sequence repeat (ISSR) molecular markers and pathogenicity assays were used for analysis the population genetic structure and pathogenicity of 7 C. heterostrophus populations (Nanping, Ningde, Fuzhou, Sanming, Putian, Longyan, Zhangzhou) from sweet corn in Fujian province. A total of 198 loci were detected using optimized 13 ISSR primers, and the percentage of polymorphic DNA loci (PL) were 100.0%, with the highest level of DNA polymorphism being detected from Fuzhou population (PL=73.7%) and the lowest level of DNA polymorphism being detected from those in Longyan (LY) and Zhangzhou (ZZ) populations (58.6% and 56.6%). ISSR marker results indicated that a total of 126 multilocus haplotypes were detected among C. heterostrophus populations from the 7 geographical locations in Fujian province, with no shared multilocus haplotypes being detected from these populations. The most abundant multilocus haplotype diversity (HS) was detected from Nanping (NP) population (HS=0.323), whereas the lowest diversity was detected from those in LY (HS=0.157). A low genetic differentiation was detected between C. heterostrophus populations from NP and Ningde, Putian and Sanming, and LY and ZZ (ΦPT<0.088), with the frequent gene flow being detected between these populations (Nm>5). However, moderate to high genetic differentiation was detected among populations in the other geographical locations. One hundred and twenty-six multilocus haplotype isolates from sweet corn in Fujian province were divided into 8 genetic clusters at genetic similarity coefficient of 0.768, with different isolates originated from the same location clustering together. The analysis of molecular variance (AMOVA) of 7 C. heterostrophus populations indicated that approximately 81.9% and 18.1% of the total variation occurred within and among populations, respectively. This result revealed that the major source of genetic variation in Fujian C. heterostrophus population was derived from within populations. The principal coordinates (PCoA) and population structure analysis showed that Fujian C. heterostrophus population could be divided into 2 genetic groups. Pathogenicity assays indicated that the 7 regional populations of C. heterostrophus had higher pathogenicity to 4 resistant and susceptible sweet corn cultivars, with the average disease index ranging from 36.42 to 59.23. Analysis of variance showed highly significant differences in pathogenicity among isolate, location, and cultivar, respectively (P<0.001). Nevertheless, no significant differences in pathogenicity among isolate×cultivar (P=0.999) and location×cultivar (P=0.361) interactions were detected at the level of 99%. This study provides a theoretical reference for the further investigation of genetic variation of C. heterostrophus and the resistance breeding efforts.
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Received: 26 April 2021
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Corresponding Authors:
* yxjzb@126.com
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