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| Cloning of ZlRPM1.1 and ZlRPM1.2 Genes and Responses to the Hyphal Infection of Ustilago esculenta in Jiaobai |
| YAN Mu-Xi*, CUI Hai-Feng*, LI Shuai, ZHAO Jin-Lan, YU Yue, ZHANG Ya-Fen, YU Xiao-Ping, YE Zi-Hong** |
| Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China |
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Abstract As an important aquatic vegetable in China, Jiaobai was the swollen stem of Zizania latifolia induced by hyphal infection of Ustilago esculenta. Resistance to pseudomonas maculicola 1 (RPM1), as a disease resistance related gene, can recognize the invasion signal of pathogens and activate the defense response of plants. In this study, 2 genes, ZlRPM1.1 (GenBank No. KP 729626) and ZlRPM1.2 (GenBank No. KP 729627) in Jiaobai responding to hyphal infection of U. esculenta were cloned by the methods of rapid amplification of cDNA ends (RACE). Alignment analysis of amino acid sequence predicted that the 2 genes belonged to CC-NBS-LRR (coiled coil-nucleotide binding site-leucine rich repeat) genes related to plant disease resistance, and were similar to the genes in Oryza sativa as high as 61% (ZlRPM1.1) and 79% (ZlRPM1.2), respectively. After artificial infection of U. esculenta in the plantlets of Z. latifolia, qPCR analysis of the stem showed that hyphae infection of U. esculenta could significantly induce the enhanced expression of ZlRPM1.1 and ZlRPM1.2 in Z. latifolia. The expression of the 2 ZlRPM1 genes among different swollen stems showed that the grey Jiaobai had significantly higher expression than that in normal Jiaobai, which might be related to the ability of hyphae infection between MT and T type of U. esculenta. During the development of swollen stem in normal Jiaobai, the expression of the 2 ZlRPM1 genes were significantly induced at the stem of mid- and late development stages. The highest expression was found at the swollen stem which was expanded to the length of 15 cm. There was significantly increased expression of ZlRPM1.2 gene in the initial swollen stem at the stage of 8-leaf, following with the higher expression in the stem than that in the leaf. However, no significant increased expression of ZlRPM1.1 was detected at initial stage of stem swelling, and the expression in the leaf was significantly higher than that in the stem. Above results indicate that both the 2 ZlRPM1 genes were involved in the formation of swollen stem induced by the infection of U. esculenta, and the ZlRPM1.2 gene might be an important regulatory gene involved in the response of stem in Z. latifolia to hyphal infection. The present study provides a reference for further investigation on the regulation mechanism of Z. latifolia plants in response to hyphal infection and stem swelling.
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Received: 18 January 2021
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Corresponding Authors:
** zhye@cjlu.edu.cn * These authors contributed equally to this work
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[1] 金晔. 2015. 茭白茎部膨大前后应激响应的蛋白质组分析[D]. 硕士学位论文, 中国计量大学, 导师: 叶子弘, pp. 49-50.
(Jin Y. 2015. Proteomic analysis of the stress response in pre- and post-swollen stem in Zizania latifolia[D]. Thesis for M.S., China Jiliang University, Supervisor: Ye Z H, pp. 49-50.)
[2] 康璐瑶, 邢阿宝, 崔海峰, 等. 2017. ZlADR1基因的克隆及其在茭白孕茭中的表达[J]. 农业生物技术学报, 25(11): 1799-1808.
(Kang L Y, Xing A B, Cui H F, et al. 2017. Cloning and expression of ZlADR1 gene during the formation of swollen stem of Zizania latifolia[J]. Journal of Agricultural Biotechnology, 25(11): 1799-1808.)
[3] 李帅. 2016. 茭白ZlRPM1和ZlADR1抗病基因的克隆及表达分析[D]. 硕士学位论文, 中国计量大学, 导师:叶子弘, pp.1-2.
(Li S. 2016. Cloning and expression analysis of ZlRPM1 and ZlADR1 resistance genes in Zizania latifolia[D]. Thesis for M. S., China Jiliang University, Supervisor: Ye Z H, pp. 1-2.)
[4] 施国新, 徐祥生. 1991. 茭白黑粉菌在茭白植株内形态发育的初步研究[J]. 云南植物研究, 73(2): 167-173.
(Shi G X, Xu X S. 1991. A preliminary study on the morphogenetic development of Ustilago esculenta in plant of Zizania caduciflora[J]. Acta Botanica Yunnanica, 73(2): 167-173.)
[5] 石敏, 黄建中, 蒋琴素, 等. 2013. 茭白黑粉菌耐热突变体的辐射诱变选育与生长特征研究[J]. 长江蔬菜, (18): 95-97.
(Shi M, Huang J Z, Jiang Q S, et al. 2013.Selection of thermo-tolerant mutants of Ustilago esculenta by irradiation and their growth characteristics[J]. Journal of Changjiang Vegetables, (18): 95-97.)
[6] 殷淯梅, 张雅芬, 曹乾超, 等. 2019. 茭白人工孕茭体系的建立及优化[J]. 农业生物技术学报, 27(8): 1498-1512.
(Yin Y M, Zhang Y F, Cao Q C, et al. Establishment and optimization of the artificial system for the formation of Jiaobai[J]. Journal of Agricultural Biotechnology, 27(8): 1498-1512.)
[7] 应荣, 崔海峰, 倪方群, 等. 2014. 杀菌剂敌磺钠及植物生长调节剂对茭白孕茭的影响[J]. 植物生理学报, 50(7): 946-952.
(Ying R, Cui H F, Ni F Q, et al. 2014. Effects of fungicide fenaminosulf and plant growth regulator on gall formation of Zizania latifolia[J]. Plant Physiology Journal, 50(7): 946-952.)
[8] 张聂, 寿森炎. 2006. 茭白孕茭的生理变化研究[J]. 浙江农业科学, 06: 613-617.
(Zhang N, Shou S Y. 2006. Study on the physiological changes of Zizania latifolia[J]. Journal of Zhejiang Agricultural Sciences, 06: 613-617.)
[9] 赵金兰, 周美琪, 叶子弘, 等. 2019. ZlGH3-8基因克隆及其在菰发育过程中的表达分析[J]. 农业生物技术学报, 27(11): 1942-1950.
(Zhao J L, Zhou M Q, Ye Z H, et al. 2019. Cloning of ZlGH3-8 Gene and its expression analysis in Zizania latifolia during development[J]. Journal of Agricultural Biotechnology, 27(11): 1942-1950.)
[10] 周美琪, 李帅, 崔海峰, 等. 2018. 菰ZlWRKY72转录因子克隆及其在茎部膨大发育中的表达分析[J]. 农业生物技术学报, 26(06): 920-930.
(Zhou M Q, Li S, Cui H F, et al. 2018. Cloning of ZlWRKY72 transcription factor and its expression analysis during the development of swollen stem of Zizania latifolia[J]. Journal of Agricultural Biotechnology, 26(06): 920-930.)
[11] Armijo G, Salinas P, Monteoliva M I, et al.2013. A salicylic acid-induced lectin-like protein plays a positive role in the effector-triggered immunity response of Arabidopsis thaliana to Pseudomonas syringae Avr-Rpm1[J]. Molecular Plant-Microbe Interactions, 26(12): 1395-1406.
[12] Belkhadir Y, Nimchuk Z, Hubert D A, et al.2004. Arabidopsis RIN4 negatively regulates disease resistance mediated by RPS2 and RPM1 downstream or independent of the NDR1 signal modulator and is not required for the virulence functions of bacterial type Ⅲ effectors AvrRpt2 or AvrRpm1[J]. The Plant Cell, 16(10): 2822-2835.
[13] Doehlemann G, Wahl R, Vranes M, et al.2008. Establishment of compatibility in the Ustilago maydis/maize pathosystem[J]. Journal of Plant Physiology, 165(1): 29-40.
[14] Geng X, Shen M, Kim J H, et al.2016. The Pseudomonas syringae type Ⅲ effectors AvrRpm1 and AvrRpt2 promote virulence dependent on the F-box protein COI1[J]. Plant Cell Reports, 35(4): 1-12.
[15] Guo H B, Li S M, Peng J, et al.2007. Zizania latifolia Turcz cultivated in China[J]. Genetic Resources & Crop Evolution, 154(6): 1211-1217.
[16] Guo L, Qiu J, Han Z, et al.2015. A host plant genome (Zizania latifolia) after a century-long endophyte infection[J]. Plant Journal, 83(4): 600-609.
[17] Leister R T, Ausubel F M, Katagiri F.1996. Molecular recognition of pathogen attack occurs inside of plant cells in plant disease resistance specified by the Arabidopsis genes RPS2 and RPM1[J]. Proceedings of the National Academy of Sciences of the USA, 93(26): 15497-15502.
[18] Li M, Ma X, Chiang Y H, et al.2014. Proline isomerization of the immune receptor-interacting protein RIN4 by a cyclophilin inhibits effector-triggered immunity in Arabidopsis[J]. Cell Host & Microbe,16(4): 473-483.
[19] Li Z, Huang J, Wang, Z, et al.2019. Overexpression of Arabidopsis nucleotide-binding and leucine-rich repeat genes rps2 and rpm1 (d505v) confers broad-spectrum disease resistance in rice[J]. Frontiers in Plant Science, 10: 417.
[20] Liu Q, You W Y, Yu X P, et al.2010. A preliminary study on proteome variations associated with gall formation in Zizania latifolia Trucs[J]. Plant Molecular Biology Reports. 29(2): 360-368.
[21] Marone D, Russo M A, Laidò G, et al.2013. Plant nucleotide binding site-leucine-rich repeat (NBS-LRR) genes: Active guardians in host defense responses[J]. International Journal of Molecular Sciences, 14(4): 7302-7326
[22] Peng Y J, Wersch R V, Zhang Y L, et al.2018. Convergent and divergent signaling in PAMP-triggered immunity and effector-triggered immunity[J]. Molecular Plant-Microbe Interactions, 31(4): 403-409.
[23] Ramirez-Prado J S, Abulfaraj A A, Naganand R, et al.2018. Plant immunity: From signaling to epigenetic control of defense[J]. Trends in Plant Science, 23(9): 833-844.
[24] Wang M C, Zhao S W, Zhu P L, et al.2018. Purification, characterization and immunomodulatory activity of water extractable polysaccharides from the swollen culms of Zizania latifolia[J]. International Journal of Biological Macromolecules: Structure, Function and Interactions, 107: 882-890.
[25] Wang Z D, Yan N, Wang Z H, et al.2017. RNA-seq analysis provides insight into reprogramming of culm development in Zizania latifolia induced by Ustilago esculenta[J]. Plant Molecular Biology, 95(6): 533-547
[26] Yan N, Wang X Q, Xu X F, et al.2013. Plant growth and photosynthetic performance of Zizania latifolia are altered by endophytic Ustilago esculenta infection[J]. Physiological & Molecular Plant Pathology, 83: 75-83.
[27] Ye Z H, Pan Y, Zhang Y F, et al.2017. Comparative whole-genome analysis reveals artificial selection effects on Ustilago esculenta genome[J]. DNA Research, 24(6): 635-648.
[28] Zhang J Z, Chu F Q, Guo D P, et al.2012. Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia[J]. Mycological Progress, 11(2): 499-508.
[29] Zhang J Z, Chu F Q, Guo D P, et al.2014. The vacuoles containing multivesicular bodies: A new observation in interaction between Ustilago esculenta and Zizania latifolia[J]. European Journal of Plant Pathology, 138: 79-91.
[30] Zhang Y, Cao Q, Hu P, et al.2017. Investigation on the differentiation of two Ustilago esculenta strains-implications of a relationship with the host phenotypes appearing in the fields[J]. BMC Microbiology, 17(1): 228. |
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