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| Selection and Application of Reference Genes for qRT-PCR in Ustilago esculenta |
| GE Xin-Tao, YIN Yu-Mei, GAO Li-Dan, HU Ying-Li, XIA Wen-Qiang, YE Zi-Hong, TANG Jin-Tian, ZHANG Ya-Fen* |
| College of Life Sciences, China Jiliang University, Hangzhou 310018, China |
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Abstract Wild rice stem is the expanded edible fleshy stem of Zizania latifolia, a Gamineous plant, when it is invaded by Ustilago esculenta. The selection of appropriate internal reference genes is important for analyzing the related gene expression profile in the study of the interaction between Zizania latifolia and Ustilago esculenta. In this study, the expression profiles of 8 candidate genes in U. esculenta were evaluated by qRT-PCR under different environmental conditions including 4 temperatures, 3 carbon source media, 5 developmental stages in vitro, and 5 time points during infection process. Data were analyzed using geNorm, NormFinder and BestKeeper software. In order to test the stability of the selected genes, the endoglucanase 1 gene (UeEgl1) was used as the test gene. The results showed that glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) and α-tubulin expressed stably in all samples and were suitable for expression pattern analysis of U. esculenta. The results of this study could provide an effective correction tool for gene differential expression analysis of U. latifolia, ensure the accuracy and reliability of gene expression results, and provide reference for further analysis of the function of related genes in the process of interaction between U. latifolia and Z. latifolia.
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Received: 19 June 2020
Published: 01 February 2021
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Corresponding Authors:
* yfzhang@cjlu.edu.cn
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