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The Characteristics of LH and PRL Hormones Changes in Small Tail Han Sheep (Ovis aries) and Sunite Sheep from Short-photoperiod to Long-photoperiod |
XIA Qing1, *, ZHANG Jin-Long2, *, DI Ran1, HE Xiao-Yun1, ZHANG Xiao-Sheng2, WANG Xiang-Yu1, HU Wen-Ping1, LIU Qiu-Yue1, **, CHU Ming-Xing1, ** |
1 Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture / Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2 Tianjin Institute of Animal Science, Tianjin 300381, China |
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Abstract The majority of sheep (Ovis aries) in China are seasonal estrus, and the trait of estrus is regulated by hypothalamic-pituitary-gonadal axis related hormones. For exploring the secretion changes of luteinizing hormone (LH) and prolactin (PRL) in year-round estrous Small Tail Han (STH) sheep and seasonal estrous Sunite sheep at different photoperiods can contribute to understand the molecular mechanism of seasonal estrus in the term of hormone. In this study, the ovariectomized Small Tail Han sheep and Sunite sheep (SNT)were raised in light-controlled sheep house, keeping in short-photoperiod (SP) condition for 42 d, then transformed to long-photoperiod (LP) condition for 42 d, and jugular vein blood was collected in different light conditions. Radioimmunoassay was used to detect the concentration of LH and PRL in 2 breeds of sheep at different photoperiods. The results showed that the concentration of PRL in the blood of Sunite sheep was lower in short-photoperiod than that in long-photoperiod, and it was basically stable in different photoperiods in Small Tail Han sheep. It was significantly higher in Small Tail Han sheep than that in Sunite sheep at SP7 and SP21 (P<0.05). The concentration of PRL in Small Tail Han sheep was significantly lower than that in Sunite sheep after LP28 (P<0.05). The concentration of LH in the blood of Small Tail Han sheep was basically stable in different photoperiods. The concentration of LH in Sunite sheep was significantly lower than that in Small Tail Han sheep at SP21 (P<0.05). In summary, the change of PRL concentration in long-photoperiod and short-photoperiod was an important factor leading to the change of estrus status in seasonal estrus sheep. It was suggested that the concentration of PRL in long-photoperiod was high in Sunite sheep, which might weaken the LH response to gonadotropin-releasing hormone (GnRH) in the pituitary of the Sunite sheep, further to inhibit the follicular maturation and ovulation, and provide basic data for further exploration of the seasonal estrus mechanism of sheep in terms of hormones.
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Received: 15 August 2019
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Corresponding Authors:
** Corresponding authors, qiuyue1983921@163.com; mxchu@263.net
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About author:: * The authors who contributed equally |
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1 贺建宁, 王金鑫, 狄冉, 等. 2013. 常年发情和季节性发情绵羊在不同季节生殖激素变化规律[J]. 畜牧兽医学报, 44(10): 1547-1553.
(He J N, Wang J X, Di R, et al.2013. Seasonal variations of reproductive hormone level in year-round and seasonal estrus sheep[J]. Acta Veterinaria et Zootechnica Sinica, 44(10): 1547-1553.)
2 田占伟, 赵宗胜, 林杉, 等. 2016. 新疆哈萨克绵羊在乏情期和发情期生殖激素的变化规律[J]. 江苏农业科学, 44(2): 256-259.
(Tian Z W, Zhao Z S, Lin B, et al.2016. Changes of reproductive hormones in Xinjiang Kazakh sheep during estrus and estrus[J]. Jiangsu Agricultural Sciences, 44(2): 256-259.)
3 夏青, 刘秋月, 王翔宇, 等. 2018. 绵羊季节性繁殖分子机制及休情季节诱导绵羊发情配种技术[J]. 遗传, 40(5): 369-377.
(Xia Q, Liu Q Y, Wang X Y, et al.2016. The molecular mechanism of sheep seasonal breeding and artificial regulatory techniques for estrus and mating in anestrus[J]. Hereditas, 40(5): 369-377. )
4 张小辉. 2004. MLT、LH和FSH对绵羊季节性发情的调控作用研究[D]. 硕士学位论文, 西北农林科技大学, 导师: 陈玉林, pp. 21-28.
(Zhang X H.2004. The regulation of melatonin, LH and FSH on the seasonal oestrus of sheep[D]. Thesis for M.S., Northwest A&F University, Supervisor: Chen Y L, pp. 21-28.)
5 Biernat W, Kirsz K, Szczesna M, et al.2018. Resistin regulates reproductive hormone secretion from the ovine adenohypophysis depending on season[J]. Domestic Animal Endocrinology, 65: 95-100.
6 Billings H J, Vigui C, Karsch F J, et al.2002. Temporal requirements of thyroid hormones for seasonal changes in LH secretion[J]. Endocrinology, 143(7): 2618-2625.
7 Christian H C, Imirtziadis L, Tortonese D.2015. Ultrastructural changes in lactotrophs and folliculo-stellate cells in the ovine pituitary during the annual reproductive cycle[J]. Journal of Neuroendocrinology, 27(4): 277-284.
8 Dardente H, Wyse C A, Birnie M J, et al.2010. A molecular switch for photoperiod responsiveness in mammals[J]. Current Biology, 20(24): 2193-2198.
9 Di R, He J, Song S, et al.2014. Characterization and comparative profiling of ovarian microRNAs during ovine anestrus and the breeding season[J]. BMC Genomics, 15(1): 899.
10 Fleisch A, Bollwein H, Piechotta M, et al.2015. Reproductive performance of Lacaune dairy sheep exposed to artificial long days followed by natural photoperiod without and with additional progestagen treatment during the nonbreeding season[J]. Theriogenology, 83(3): 320-325.
11 Gam S, De Ruediger F R, Zundt M, et al.2018. Characterization of the LH peak after short and long fixed-time artificial insemination protocols in sheep raised in the tropics[J]. Journal of Animal Science, 89(9): 1245-1252.
12 Gómezbrunet A, Santiagomoreno J, Malpaux B, et al.2012. Ovulatory activity and plasma prolactin concentrations in wild and domestic ewes exposed to artificial photoperiods between the winter and summer solstices[J]. Animal Reproduction Science, 132(1-2): 36-43.
13 Grazul-Bilska A T, Reyaz A, Valkov V, et al.2018. Follicle stimulating hormone receptor protein is expressed in ovine uterus during the estrous cycle and utero-placenta during early pregnancy: An immunohistochemical study[J]. Acta Histochemica, 120(5): 420-428.
14 Gregory S J, Townsend J, Mcneilly A S, et al.2004. Effects of prolactin on the luteinizing hormone response to gonadotropin- releasing hormone in primary pituitary cell cultures during the ovine annual reproductive cycle[J]. Biology of Reproduction, 70(5): 1299-1305.
15 Hanon E A, Lincoln G A, Fustin J M, et al.2008. Ancestral TSH mechanism signals summer in a photoperiodic mammal[J]. Current Biology, 18(15): 1147-1152.
16 Hazlerigg D, Lomet D, Lincoln G, et al.2018. Neuroendocrine correlates of the critical day length response in the Soay sheep[J]. Journal of Neuroendocrinology, 30(9): e12631.
17 Hodson D J, Henderson H L, Townsend J, et al.2012. Photoperiodic modulation of the suppressive actions of prolactin and dopamine on the pituitary gonadotropin responses to gonadotropin-releasing hormone in sheep[J]. Biology of Reproduction, 86(4): 122.
18 Hodson D J, Townsend J, Gregory S J, et al.2010. Role of prolactin in the gonadotroph responsiveness to gonadotrophin-releasing hormone during the equine annual reproductive cycle[J]. Journal of Neuroendocrinology, 22(6): 509-517.
19 Hut R A, Dardente H, Riede S J.2014. Seasonal timing: how does a hibernator know when to stop hibernating?[J]. Current Biology, 24(13): 602-605.
20 Iurilli G, Ghezzi D, Olcese U, et al.2012. Sound-driven synaptic inhibition in primary visual cortex[J]. Neuron, 73(4): 814-828.
21 Jackson G L, Davis S L.1979. Comparison of luteinizing hormone and prolactin levels in cycling and anestrous ewes[J]. Neuroendocrinology, 28(4): 256-263.
22 Lincoln G A.2006. Decoding the nightly melatonin signal through circadian clockwork[J]. Molecular and Cellular Endocrinology, 252(1-2): 69-73.
23 Lincoln G A, Klandorf H, Anderson N.1980. Photoperiodic control of thyroid function and wool and horn growth in rams and the effect of cranial sympathectomy[J]. Endocrinology, 107(5): 1543-1548.
24 Liu Z, Ji Z, Wang G, et al.2016. Genome-wide analysis reveals signatures of selection for important traits in domestic sheep from different ecoregions[J]. BMC Genomics, 17(1): 863.
25 Masumoto K H, Ukaitadenuma M, Kasukawa T, et al.2010. Acute induction of Eya3 by late-night light stimulation triggers TSHβ expression in photoperiodism[J]. Current Biology, 20(24): 2199-2206.
26 Mccosh R B, Szeligo B M, Bedenbaugh M N, et al.2017. Evidence that endogenous somatostatin inhibits episodic, but not surge, secretion of LH in female sheep[J]. Endocrinology, 158(6): 1827-1837.
27 Mekuriaw Z, Assefa H, Tegegne A, et al.2016. Estrus response and fertility of Menz and crossbred ewes to single prostaglandin injection protocol[J]. Tropical Animal Health and Production, 48(1): 53-57.
28 Menassol JB, Collet A, Chesneau D, et al.2012. The interaction between photoperiod and nutrition and its effects on seasonal rhythms of reproduction in the ewe[J]. Biology of Reproduction, 86(2): 52.
29 Misztal T, Romanowicz K, Tomaszewska Z D, et al.2004. The effects of prolonged, intracerebroventricular prolactin treatment on luteinizing hormone secretion, catecholaminergic activity and estrous behavior in ewes[J]. Experimental and Clinical Endocrinology & Diabetes, 112(4): 215-221.
30 Morgan.2000. The pars tuberalis: The missing link in the photoperiodic regulation of prolactin secretion?[J]. Journal of Neuroendocrinology, 12(4): 287-295.
31 Picazo R A, Gonzalez D B A, Gomez B A, et al.2000. Effects of bromocriptine administration during the follicular phase of the oestrous cycle on prolactin and gonadotrophin secretion and follicular dynamics in merino monovular ewes[J]. Journal of Reproduction & Fertility, 120(1): 177-186.
32 Szczesna M, Kirsz K, Misztal T, et al.2018. The effects of leptin on plasma concentrations of prolactin, growth hormone, and melatonin vary depending on the stage of pregnancy in sheep[J]. Journal of Animal Science, 96(8): 3348-3357 .
33 Tortonese D J.2016. Intrapituitary mechanisms underlying the control of fertility: Key players in seasonal breeding[J]. Domestomestic Animal Endocrinology, 56(Suppl): 191-203.
34 Tortonese D J, Brooks J, Ingleton P M, et al.1998. Detection of prolactin receptor gene expression in the sheep pituitary gland and visualization of the specific translation of the signal in gonadotrophs[J]. Endocrinology, 139(12): 5215-5223.
35 Tsujino K, Narumi R, Masumoto K H, et al.2013. Establishment of TSHβ real-time monitoring system in mammalian photoperiodism[J]. Genes Cells, 18(7): 575-588.
36 Walton J S, Mcneilly J R, Mcneilly A S, et al.1977. Changes in concentrations of follicle-stimulating hormone, luteinizing hormone, prolactin and progesterone in the plasma of ewes during the transition from anoestrus to breeding activity[J]. Journal of Endocrinology, 75(1): 127-136.
37 Wernerkorf H.2018. Signaling pathways to and from the hypophysial pars tuberalis, an important center for the control of seasonal rhythms[J]. General and Comparative Endocrinology, 258: 236-243.
38 Wood S H, Christian H C, Miedzinska K, et al.2015. Binary switching of calendar cells in the pituitary defines the phase of the circannual cycle in mammals[J]. Current Biology, 25(20): 2651-2662.
39 Wood S, Loudon A.2014. Clocks for all seasons: Unwinding the roles and mechanisms of circadian and interval timers in the hypothalamus and pituitary[J]. Journal of Endocrinology, 222(2): R39-R59.
40 Zhai M, Xie Y, Liang H, et al.2018. Comparative profiling of differentially expressed microRNAs in estrous ovaries of kazakh sheep in different seasons[J]. Gene, 664: 181-191. |
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