Correlation Analysis of UCP3 Gene Variation, Expression and Meat Quality Traits in Jiangkou Luobo Pig (Sus scrofa)
ZHANG Ji1, WU Xue1, QIU Gan-Yuan1, LIU Peng-Cheng1, YANG Mao-Lin2, LIU Ruo-Yu1, *
1 College of Animal Sciences, Guizhou University/Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang 550025, China;
2 Science and Technology Bureau of Jiangkou County, Tongren 554400, China
Abstract Uncoupling protein 3 (UCP3), as an important regulatory protein, is mainly involved in the regulation of the body's energy metabolism and plays an important role in lipid metabolism, fatty acid oxidation and fat deposition. It plays a decisive role in the production of ATP in oxidative phosphorylation. In order to study the relationship between UCP3 gene and pork quality traits, 11 meat quality indexes of 165 Jiangkou Luobo pigs (Sus scrofa) were determined. SNPs loci were screened by sequencing all exons of UCP3 gene amplified by mixed DNA pool. Single sample DNA was amplified and sequenced for validation and typing. At the same time, the difference of meat quality among individuals with different genotypes was analyzed. The expression of UCP3 gene of individuals with different genotypes was detected to study the relationship between meat quality and UCP3 gene expression. This study identified 6 SNPs, they were g.3127A>G, g.3205T>C, g.10439C>T, g.10441C>A, g.10466G>A and g.10479A>G/T. Population genetic parameters analysis showed that the heterozygosity except g.10479A>G/T locus was high , which indicated that the distribution of alleles in the population was relatively uniform; the number of effective alleles was high, which indicated that the degree of variation in the population was large; and the content of polymorphic information was more than 0.25, which indicated that the loci could provide some genetic information. χ2 test showed that the genotype distribution of g.3127A>G and g.3205T>C loci was in Hardy-Weinberg equilibrium, while the genotype distribution of g.10439C>T, g.10441C>A and g.10466G>A loci deviated significantly from Hardy-Weinberg equilibrium. Linkage disequilibrium analysis showed that g.3127A>G and g.3205T>C loci, g.10439C>T and g.10466G>A loci were completely linkage disequilibrium, g.10441C>A, g.10466G>A and g.10479A>G/T were strongly linkage disequilibrium. The correlation analysis between SNPs loci and meat quality showed that marbling scores of TT and GG individuals at g.10479A>G/T loci were significantly higher than those of AA and AG individuals, whereas shear stress was the opposite. Genotype-expression correlation analysis showed that g.10439C>T, g.10441C>A, g.10466G>A and g.10479A>G/T loci were significantly higher than those of others. UCP3 gene expression was negatively correlated with shear stress and positively correlated with intramuscular fat content. The results showed that g.10439C>T, g.10441C>A, g.10466G>A and g.10479A>G/T sites were important SNPs sites affecting the meat quality of Jiangkou Luobo pigs, and the increase of UCP3 gene expression was beneficial to the deposition of intramuscular fat. The results provide a reference for the screening of molecular markers that contribute to intramuscular fat deposition in Jiangkou Luobo pig.
1 陈哲. 2011. 猪SIM1、PPARγ2和UCP3基因分子特性及遗传效应研究[D]. 硕士学位论文, 浙江大学,导师: 徐宁迎, pp. 111-112.
(Chen Z.2011. The characterizations and genetic effect of porcine SIM1, PPARγ2 and UCP3 Genes[D]. Thesis for M.S., Zhejiang University, Supervisor: Xu N Y, pp. 111-112.)
2 陈哲, 于峰祥, 于建宁, 等. 2012. 猪UCP3基因第4内含子BccⅠ多态性检测及其与胴体和肉质性状的关联分析[J]. 江苏农业学报, 28(06): 1338-1343.
(Chen Z, Yu F X, Yu J N, et al.2012. Detection of BccⅠ polymorphism in intron 4 of porcine gene and its correlation with carcass and meat quality traits[J]. Jiangsu Journal of Agricultural Sciences, 28(06): 1338-1343.)
3 方美英, 赵兴波, 吴常信, 等. 2002. 不同品种猪解耦联蛋白基因3'调控区的遗传变异[J]. 科学通报, 47(13): 1010-1012.
(Fang M Y, Zhao X B, Wu C X, et al.2002. Genetic variation of the 3' regulatory region of the pig's uncoupling protein gene in different varieties[J]. Chinese Science Bulletin, 47(13): 1010-1012.)
4 龚建军. 2005. 德国猪肉品质测定技术[J]. 中国畜牧兽医, 32(9): 23-26.
(Gong J J.2005. German pork quality measurement technology[J]. China Animal Husbandry and Veterinary Medicine, 32(9): 23-26.)
5 胡倩. 2008. 贵州地方猪种肉质功能基因的研究[D]. 硕士学位论文, 贵州大学, 导师: 王嘉福, pp. 54-56.
(Hu Q.2008. Study of function gene to meat quality performance in Guizhou local porcine[D]. Thesis for M.S., Guizhou University, Supervisor: Wang J F, pp. 54-56.)
6 黄爱霞. 2011. 浙江省主要鸡种肉品质分析及Leptin receptor基因对鸡脂肪代谢影响分子机制的研究[D]. 硕士学位论文, 浙江大学, 导师: 吴天星, pp. 75-76.
(Huang A X.2011. Analysis of chicken meat quality in Zhejiang province and study on molecular mechanism of leptin receptor gene in lipid metabolism[D]. Thesis for M.S., Zhejiang University, Supervisor: Wu T X, pp. 75-76.)
7 李秋玲, 许尚忠, 昝林森, 等. 2006. 3个牛品种UCP3基因第5内含子和第6外显子部分序列的多态性研究[J]. 西北农林科技大学学报(自然科学版), 34(11): 11-20.
(Li Q L, Xu S Z, Zan L S, et al.2006. Study on polymorphisms of part sequence of intron 5 and exon 6 of bovine UCP3 gene[J]. Journal of Northwest A&F University(Nature Science Edition), 34(11): 11-20.)
8 李艳华, 郦汉杰, 赵兴波, 等. 2006. 猪UCP2基因5'调控区和外显子1的遗传变异研究[J]. 生物化学与生物物理进展, 33(3): 262-266.
(Li Y H, Li H J, Zhao X B, et al.Research on genetic variation of 5' regulatory region and exon 1 of UCP2 in pigs[J]. Progress in Biochemistry and Biophysic, 33(3): 262-266.)
9 刘建华. 2013. 绵羊UCP2和UCP3基因的克隆、表达及其遗传变异研究[D]. 山西农业大学, 导师: 刘文忠, pp. 142-143.
(Liu J H.2013. Study on the cloning, expression and genetic variation of ovine UCP2 and UCP3 genes[D]. Shanxi Agricultural University, Supervisor: Liu W Z, pp. 142-143.)
10 彭广南. 2013. 广西三黄鸡PPARγ和UCP3基因表达水平与脂肪性状的相关研究[D]. 硕士学位论文, 广西大学, 导师: 郭亚芬, pp. 62-66.
(Peng G N.2013. Corelation analysis between expression levels of PPARγ and UCP3 gene and meat quality traits in Guangxi Sanhuang chicken[D]. Thesis for M.S., Guangxi University, Supervisor: Guo Y F, pp. 62-66.)
11 任亮, 朱宝芹, 韩丹, 等. 2006. 猪UCP3基因多态性及其与脂肪沉积、肉质性状的相关分析[J]. 农业生物技术学报, 14(5), 652-656.
(Ren L, Zhu B Q, Han D, et al.2006. Polymorphism of porcine uncoupling protein 3 (UCP3) and its association with fat deposition and meat quality[J]. Journal of Agricultural Biotechnology, 14(5): 652-656.)
12 涂荣剑. 2003. 猪解耦联蛋白基因3 (UCP3)的序列及多态性研究[D]. 华中农业大学, 导师: 邓昌彦, pp. 39-41.
(Tu R J.2003. Research on sequence and polymorphism of porcine uncoupling protein gene 3[D]. Huazhong Agricultural University, Supervisor: Deng C Y, pp. 39-41.)
13 王阳, 冉雪琴, 王嘉福, 等. 2011. 糯谷猪解耦联蛋白3和心脏型脂肪酸结合蛋白在骨骼肌中的表达和定位[J]. 中山大学学报(自然科学版), 50(01): 99-103.
(Wang Y, Ran X Q, Wang J F, et al.2001. Expression and localization of uncoupling protein 3 and heart fatty acid-binding protein in skeletal[J]. ACTA Scientiarum NatUralium Universitatis Sunyatseni, 50(01): 99-103.)
14 伍革民, 许晓风, 章熙霞, 等. 2007. 山猪UCP3基因遗传变异及其与胴体、肉质性状的相关研究[J]. 江苏农业科学, (04): 132-135.
(Wu G M, Xu X F, Zhang X X, et al. 2007. Study on genetic variation of UCP3 gene and its correlation with carcass and meat quality traits in mountain pigs[J]. Jiangsu Agricultural Science, (04): 132-135.)
15 张雷, 文生萍, 谷大海, 等. 2010. UCPs基因多态性与脂肪代谢的相关性研究[J]. 中国畜牧兽医, 37(8): 78-83.
(Zhang L, Wen S P, Gu D H, et al.2010. The polymorphisms of UCPs genes associated with fat metabolism[J]. China Animal Husbandry and Veterinary Medicine, 37(8): 78-83.)
16 Aguer C, Piccolo B D, Fiehn O, et al.2016. A novel amino acid and metabolomics signature in mice overexpressing muscle uncoupling protein 3[J]. The FASEB Journal, 31(2): 814-827.
17 Argyropoulos G, Brown A M, Willi S M, et al.1998. Effects of mutations in the human uncoupling protein 3 gene on the respiratory quotient and fat oxidation in severe obesity and type 2 diabetes[J]. Journal of Clinical Investigation, 102(7): 1345-1351.
18 Boss O, Samec S, Paolonigiacobino A, et al.1997. Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression[J]. FEBS Letters, 408(1): 39-42.
19 Joubert R, Coustard S M, Swennen Q, et al.2010. The beta-adrenergic system is involved in the regulation of the expression of avian uncoupling protein in the chicken[J]. Domestic Animal Endocrinology, 38(2): 115-125.
20 Lapice E, Monticelli A, Cocozza S, et al.2016. The combination of UCP3-55CT and PPARγ2Pro12Ala polymorphisms affects BMI and substrate oxidation in two diabetic populations[J]. Nutrition, Metabolism and Cardiovascular Diseases, 26(5): 400-406
21 Ricquier D, Frédéric B.2000. Mitochondrial uncoupling proteins: From mitochondria to the regulation of energy balance[J]. The Journal of Physiology, 529(1): 3-10.
22 Stone R T, Rexroad C E, Smith T P.2015. Bovine UCP2 and UCP3 map to BTA15[J]. Animal Genetics, 30(5): 378-381.
