Contact Us Add to Favorite
 
NianQi Search Adv Search
33
农业生物技术学报  2020, Vol. 28 Issue (3): 399-406    DOI: 10.3969/j.issn.1674-7968.2020.03.003
Articles and Letters Current Issue | Archive | Adv Search |
Effect of Ectopic Expression of AtCKX2 Gene on the Development of Tomato (Solanum lycopersicum) Fruits
LIU Xue-Xia1, LI Hu2, DONG Ying-Hua1, WU Han2, GAN Li-Jun1, *
1 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Download: PDF (2173 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  Tomato (Solanum lycopersicum) is one of the most widely grown vegetables in the world. It is a model crop for studying the development of climacteric fruits. Various plant hormones are involved in regulating the growth and development of tomato fruits. Cytokinins can promote cell division of fruit, make fruit larger, increase fruit weight, delay fruit senescence, and increase fruit yield. In order to study the effect of endogenous cytokinin on tomato fruits, the expression vector for the tomato fruit-specific promoter Tfm7 to drive the Arabidopsis thaliana cytokinin oxidase 2 gene (AtCKX2) was constructed. The transgenic plants were obtained by the method of Agrobacterium tumefaciens transformation. The transgenic plants were verified by the specific primers of the promoter Tfm7 and the target gene AtCKX2. The PCR results indicated that the promoter Tfm7 and the target gene AtCKX2 had been transferred into the tomato plants. The results of qRT-PCR showed that the AtCKX2 gene was not expressed in the leaves of tomato plants and was expressed in the pollen flowers. The expression level of AtCKX2 gene was the highest in the fruit 5 d after pollination. As the fruit developed, the expression level of AtCKX2 gene gradually decreased, and it was hardly expressed in the late stage of fruit development. Studies on tomato fruits showed that compared with wild-type tomato fruits, the content of endogenous cytokinin zeatin (ZT) and isopentenyladenine (iP) in transgenic tomato fruits decreased, the single fruit weight, vertical and horizontal diameter of transgenic fruits decreased, and the content of soluble sugar and soluble protein in fruits decreased, the content of titratable acid increased. These results indicate that endogenous cytokinins can affect the growth and development of tomato fruits. This study provides new ideas for the role of cytokinins in fruit development.
Key wordsTomato      Cytokinin      Arabidopsis thaliana cytokinin oxidase 2 (AtCKX2)      Fruit size      Fruit quality     
Received: 05 August 2019     
ZTFLH:  S641.2  
Corresponding Authors: *ganlj@njau.edu.cn   
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
LIU Xue-Xia
LI Hu
DONG Ying-Hua
WU Han
GAN Li-Jun
Cite this article:   
LIU Xue-Xia,LI Hu,DONG Ying-Hua, et al. Effect of Ectopic Expression of AtCKX2 Gene on the Development of Tomato (Solanum lycopersicum) Fruits[J]. 农业生物技术学报, 2020, 28(3): 399-406.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.03.003     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I3/399
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech