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| Expression and Protein Interactions Analysis of ZmPP2C3 Gene in Maize (Zea mays) |
| WANG Guo-Rui*, YUAN Zhen*, ZHANG Peng-Yu, QIU Xiao, LIU Zhi-Xue, WANG Tong-Chao, WEI Li** |
| College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China |
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Abstract Protein phosphates 2C (PP2C) genes play an important role in regulating plant growth and abiotic stress resistance. In this study, ZmPP2C3 (GenBank No. NM_001155565.2), screened from the previous drought-rewatering transcriptome analysis and encoding PP2C protein, was cloned in maize (Zea mays). Sequence analysis showed that the ORF of ZmPP2C3 gene was 1 227 bp, encoded 408 amino acids, and the molecular weight was 43.28 kD, isoelectric point was 5.92; Sequence alignment revealed that ZmPP2C3 shared high sequence homology with AIB05995.1 (99.75%) and AIG52088.1 (99.75%) in maize; Subcellular localization showed that the protein encoded by the ZmPP2C3 gene was detected in the nucleus. The qRT- PCR analysis showed that ZmPP2C3 gene had the highest expression in the root of corn seedlings, and the expression of ZmPP2C3 was positively regulated by drought, salt, high temperature and abscisic acid (ABA) treatment. Yeast two-hybrid analysis showed that ZmPP2C3 protein was interacted with GRMZM2G180625, GRMZM2G038126, GRMZM2G155242, GRMZM2G110408 and GRMZM2G069651. This study provides a reference for further biological function analysis of ZmPP2C3 gene.
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Received: 09 September 2019
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Corresponding Authors:
** weili-wtc@126.com
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| About author:: * The authors who contribute equally |
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