Identification and Expression Analyses of bHLH Gene Family Members in Transcriptome of Pinus massoniana Seedlings
SHANG Xian-Wen1,2, FAN Fu-Hua1,2*, ZHOU Zi-Jing1,2, QIN Hui-Juan1,2, WANG Cong-Hui1,2, DING Gui-Jie1,2*, TAN Jian-Hui3
1. Institute for Forestry Resources & Environment of Guizhou/Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou,Guizhou University,Guiyang 550025,China;
2. College of Forestry,Guizhou University,Guiyang 550025,China;
3. Guangxi Zhuang Autonomous Region Forestry Research Institue,Nanning 530002
Abstract Plant basic helix-loop-helix (bHLH) transcription factors are the second largest class of transcription factors in eukaryotes,and play important roles in plant growth,secondary metabolism and stress response. In this study,bHLH family genes were screened by transcriptome sequencing of root samples of Pinus massoniana seedlings. The subcellular location,conservative motifs,expression pattern and phylogenetic analyses of these genes were performed by bioinformatics methods. The results showed that 64 bHLH family genes were identified from the transcriptome of Pinus massoniana,and these genes were predicted to be mainly located in the nucleus. Motif1 and motif2 appeared most frequently in conserved motifs. Under the condition of low-phosphorus stress,PmbHLH01/09/27/29/32/39/41 showed significantly differential expression,suggesting that they might be involved in phosphorus stress. These results provide a reference for further revealing the biological function of bHLH transcription factors in response to phosphorus change of P. massoniana.
SHANG Xian-Wen,FAN Fu-Hua,ZHOU Zi-Jing, et al. Identification and Expression Analyses of bHLH Gene Family Members in Transcriptome of Pinus massoniana Seedlings[J]. 农业生物技术学报, 2020, 28(11): 1947-1959.
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