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| Cloning and Expression Analysis of Jasmonic Acid Carboxyl Methyltransferase Gene (JMT) in Sugarcane (Saccharum spp. hybrids) |
| CHEN Li-Lan, WANG Ya-Ru, GUO Yan-Fang, DENG Zu-Hu, WANG Jin-Da* |
| National Engineering Research Center for Sugarcane,Fujian Agriculture and Forestry University,Fuzhou 350000,China |
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Abstract Jasmonic acid substances,as endogenous regulatory hormones in plants,play important roles in plant growth and responding to stress. Jasmonic acid carboxyl methyltransferase (JMT) is a key enzyme that catalyzes synthesizing methyl jasmonate (MeJA) from jasmonate. It controls the transformation of jasmonate in plants and affects plants resistance to biological and abiotic stress. Based on sugarcane (Saccharum spp. hybrids) transcriptome database,a full-length of sugarcane jasmonate methyltransferase ScJMT (GenBank No.MK784565) was cloned and analyzed. The expression levels of ScJMT in different organs and expression profiles under different stresses were detected by qRT-PCR. Bioinformatics results showed that the open reading frame (ORF) of ScJMT gene was 1 110 bp encoding 369 aa. The isoelectric point,instability coefficient,and average hydrophobicity value of the ScJMT protein were 5.28,56.76,-0.135,respectively. The ScJMT was an unstable acid hydrophilic protein by prediction. The α-helix structure and random curl constituted its secondary structure and tertiary structure,and it also contained the methylation_7 conserved domain. Through phylogenetic tree analysis showed ScJMT was on the same branch as SvJMT from Sorghum vulgare and ZmJMT from Zea mays. In addition,the spatial expression level of ScJMT was detected and expression profiles under different stress were also determined. There were no significant differences among 3 sugarcane organs,and ScJMT was increased in abscisic acid (ABA),drought (PEG6000),high salinity treatments and oriental armyworm Mythirnna separata feeding stress,while treatments of MeJA and Acidovorax avenae subsp. avenae showed no effect on ScJMT expression. The results make foundations for further research on the role of this gene,and also provide gene resources for the cultivation of new resistant sugarcane varieties.
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Received: 24 March 2020
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Corresponding Authors:
*jdwang@fafu.edu.cn
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