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| Analysis of Complete Mitochondrial Genome and Phylogenetic Relationship of Bothrogonia ferruginea |
| YU Peng-Fei, LI Qian, WANG Meng-Xin, PAN Cheng, CUI Lin, HAN Bao-Yu* |
| Zhejiang Provincial Key Laboratory of Biometrology and Inspection &Quarantine, China Jiliang University, Hangzhou 310018, China |
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Abstract Bothrogonia ferruginea is an important agricultural and economic pest in China. Sequencing and analyzing the complete mitochondrial genome of B. ferruginea and exploring its phylogenetic status in the suborder Auchenorrhyncha at the mitochondrial level are of great significance to its systematic taxonomy. The complete mitochondrial genome sequence of B. ferruginea was obtained by PCR amplification and sequencing, and its general features and base composition were analyzed. The plyogenetic tree of mitochondrial protein-coding genes of 22 species of Auchenorrhyncha and 2 outgroups was constructed by using the Bayesian inference (BI) and maximum likelihood method (ML). The complete mitochondrial genome of B. ferruginea was 15 262 bp (GenBank No. KU167550), including 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes and one control region. The nucleotide composition and gene distribution sequence of B. ferruginea showed the same as insect of Auchenorrhyncha. The A + T content of mitochondrial genome is 76.48%, and the AT-skew is 0.170 2. There were 14 overlapping genes and 10 intergenic regions. All protein-coding genes use the ATN as initiation codon. Majority of PCGs had a complete termination codon (TAA or TAG), except that COⅡ gene terminated with the incomplete stop codon TA. All the predicted tRNAs showed the classic clover-leaf structure, except for the absence of DHU (dihydrouracil) arm of tRNASer(AGN). The A+T rich region contained some conserved sequence (ATTTA) and a typical microsatellite structure (TA)25. Two special structures, ATCTA and CCCTCT, were found in the tandem repeats in the control region of Cicadellidae, but not in the family Membracidae and other Auchenorrhyncha. The phylogenetic relationships of 22 species of Auchenorrhyncha based on mitochondrial genome were identified as Cercopoidea+(Fulgoroidea + Membracoidea). B. ferruginea, Nephotettix cincticeps, Idioscopus nitidulus, Empoasca vitis and Homalodisca vitripennis belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. The gene arrangement pattern of the mitochondrial genome of B. ferruginea was identical to those of other Cicadellidae insects. The phylogenetic relationship of Auchenorrhyncha constructed based on the mitochondrial genome was consistent with those in traditional morphological taxonomy. B. ferruginea belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. Our research provides data for the further study of auchenorrhyncha classification system.
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Received: 31 January 2019
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Corresponding Authors:
hanby15@163.com
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