花椒窄吉丁雌、雄成虫触角差异表达基因的初步筛选

doi:10.3969/j.issn.1674-7968.2019.07.011

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Abstract Agrilus zanthoxylumi is a significant and destructive branch-borers pest in the Zanthoxylum bungeanum planting areas of northern China. This insect mainly recognizes volatile odor molecules through sensitivity and specificity via antenna, which plays an important role in its survival and reproduction. To select Agrilus zanthoxylumi adult male and female antenna of differentially expressed genes, preliminary parsing and Agrilus zanthoxylumi candidate differences related to the behavior and the biological prevention and control in terms of seeking mate genes, this study selected adult female and male antenna transcriptome sequencing as the research object, using bioinformatics software to analyze the transcriptome sequencing data obtained and screening of differentially expressed genes between the 2 samples of male and female, further analysis of differentially expressed genes. The results showed that 36 209 unigene were obtained from the antenna transcriptome data of the male and female adults of Agrilus zanthoxylumi. A total of 726 genes were detected with significant differences in expression, among which 459 genes were up-expressed and 267 genes were down-expressed. Through GO (Gene Ontology) enrichment analysis, the differentially expressed genes were found in binding function, catalytic activity, cell process, metabolic process, cell components and organelles which were significantly enriched. A total of 472 differentially expressed genes were annotated in the KEGG database, and Pathway enrichment analysis showed that they were involved in 165 metabolic pathways, with 394 up-expressed and 78 down-expressed genes. This study obtained Agrilus zanthoxylumi adult antenna of the genetic basis of enrichment of the active ingredient based on the transcriptome sequencing technologies, discussed the adult male and female antenna differentially expressed genes, revealed the gene function, understood the relationship between genes. It doesn't only further improve the mechanism of tentacles feelings outside odor material plays an important role, but also provide the basic data for pest prevention and control technology.

Key words： Agrilus zanthoxylumi Transcriptome Differentially expressed genes Candidate genes Protein interaction

Received: 20 November 2018

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S763.38

Corresponding Authors: shouanxie@163.com

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	GONG Xue-Fang
	XIE Shou-An
	YANG Ping
	HU Hong-Yan
	GUO Li
	CHE Xian-Rong
	CHEN Di
	WANG Yan-Lai
	LV Shu-Jie

Cite this article:

GONG Xue-Fang,XIE Shou-An,YANG Ping, et al. Preliminary Screening of Differentially Expressed Genes of Female and Male Antenna of Agrilus zanthoxylumi[J]. 农业生物技术学报, 2019, 27(7): 1233-1245.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.07.011 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I7/1233

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