Screening of SNPs in PKMa Gene and Analysis of Its Correlations with Glucose Tolerance Trait in Ctenopharyngodon idella
FAN Jia-Jia, TANG Xiao-Hong, BAI Jun-Jie, JIANG Peng*, MA Dong-Mei, LI Sheng-Jie
Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture/Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China
Abstract Pyruvate kinase (PK) is a key rate-limiting enzyme that catalyzes the last step of glycolysis and plays an important role in animal glucose metabolism. Studies have shown that there were 2 SNPs (T+1817C and G+1818T) in the 3' non-coding region of Ma-type pyruvate kinase (PKMa) gene of Ctenopharyngodon idella. The completely linked SNPs were combined into 3 genotypes, TG/TG, CT/TG and CT/CT (abbreviated as AA, AB and BB), respectively. In order to investigate the PKMa gene polymorphism effect on glucose tolerance, the growth performance of the 3 genotypes were tested and compared in the low-glucose (LD) and high-glucose (HD) diet environments. After 8 weeks feeding trial, the results showed that the growth rate of grass carp in LD group was faster than that in HD group. In the LD population, the body weight of AA haplotype was 4.9% and 6.1% faster than AB haplotype and BB haplotype, respectively (P>0.05). In the HD population, the body weight of the AA haplotype was 36.8% and 30.4% faster than the AB haplotype and the BB haplotype, respectively (P<0.05) and increased by 6.7% compared with LD population AA haplotype. For the specific expression of PKMa gene in brain tissue, the concentration of PK enzyme in brain tissue was detected. It was shown that the PK enzyme concentration in LD population was lower than that in HD population. The AA genotype individuals was significantly higher than that of the AB and BB haplotypes individuals in the LD and HD population, respectively. The results showed that the PMKa gene mutation was associated with the body's tolerance to glucose of C. idella. It is speculated that the expression level of the PKMa enzyme concentration is increased to better utilize the glucose dietary having the AA genotype C. idella. This study provides candidate markers for the breeding of new varieties of glucose tolerant in C. idella.
FAN Jia-Jia,TANG Xiao-Hong,BAI Jun-Jie, et al. Screening of SNPs in PKMa Gene and Analysis of Its Correlations with Glucose Tolerance Trait in Ctenopharyngodon idella[J]. 农业生物技术学报, 2019, 27(6): 1072-1080.
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