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| Correlation Between Crested Traits and Hoxc8 Gene Expression and Its Growth Performance in Wumeng Crested Chickens (Gallus gallus) |
| LU Jing1, ZHANG Yong1,*, LUO Ke-Yin3, BAI You2, ZHANG Xiong4, LI Ze-Run1, WANG Tian-Song1, MO Xian-Ting2, HUANG Ming-Jie2 |
1 Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;
2 College of Animal Science/Guizhou Province Key laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, China;
3 Agricultural Committee of Liupanshui City, Liupanshui 553000, China;
4 Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang 550005, China |
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Abstract Many studies have shown that homeobox C8 (Hoxc8) gene plays an important regulatory role in the crested traits of chickens (Gallus gallus), and the crested traits have a significant impact on the early growth performance of the chickens. Therefore, in order to study the correlation between the expression of Hoxc8 gene and the crested traits and growth performance of Wumen crested chickens, this study had taken the Wumeng crested chickens as research objects, measuring the early growth performance and the expression of Hoxc8 gene in different tissues. The results showed that Hoxc8 gene was expressed in all tissues of adult crested Wumeng crested chickens. It had the highest expression in crested bone; higher expression in testis; moderate expression in kidney, chest muscle, leg muscle, tibia and large intestine; and lower expression in heart, liver, ovary, hypothalamus spleen (P<0.01); Hoxc8 gene was differentially expressed in the scalp and back shin tissues of Wumeng crested chickens with 24 weeks of age, with the slight expression in crested chickens and higher expression in non-crested chickens (P<0.01). Hoxc8 gene was differentially expressed in the tissues of female and male Wumeng crested chickens: The expression of scalp and back shin tissue in cocks of 24 weeks old Wumeng crested chickens were significantly higher than that of hens (P<0.01). In adult crested Wumeng crested chickens, the expression of tibia and leg muscle tissue of cocks was higher than that of hens, and the expression of heart, liver, hypothalamus and pituitary tissue of hens were higher than that of cocks; The crested traits had an effect on the early growth performance of Wumeng crested chickens. With the increase of the age of the week, the growth trend of the body weight, body length, chest depth, chest width and tibia length of crested Wumeng crested chickens were better than that of the non-crested Wumeng crested chickens; The early growth performance of cocks with crested and non-crested Wumeng crested chickens were better than that of hens; the early weight gain of Wumeng crested chickens with better shelling weight was better. The above results showed that the crested traits of Wumeng crested chickens were related to the specific expression of Hoxc8 gene in their scalp and back skin tissue. there was a good growth performance of crested Wumeng crested chickens. The results of this study could provide certain guiding significant for the conservation and breeding of Wumeng crested chickens.
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Received: 31 October 2018
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Corresponding Authors:
gzuzy@139.com
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