乌珠穆沁羊<em>LPL</em>基因第3外显子多态性与肉质性状的关联分析

doi:10.3969/j.issn.1674-7968.2019.03.008

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Abstract Meat quality traits are important economic value in sheep (Ovis aries) breeding. Lipoprotein lipase (LPL) is known as an important enzyme in the metabolism of lipids and lipoproteins in animal organisms, which belongs to the lipase family. LPL is mainly synthesized and secreted by adipose cells, skeletal muscle cells, myocardial cells, mammary cells and macrophages. Enzyme activity is regulated by nutritional factors and hormone levels. The A384T single nucleotide polymorphism (SNP) exists in the exon 3 of sheep LPL gene, and this SNP has been confirmed to have a certain relationship with sheep meat quality traits. Ujimqin sheep is the most important breed of Mongolia sheep population with fat-tailed, high quality meat and carpet wool, and have sound body conformation, strong walking ability and admirable adaptation to very different ecological conditions. Recently, the mutton of Ujimqin sheep is widely recognized as natural green food in China. Thus, it is necessary to improve meat quality traits of Ujimqin sheep for developing mutton sheep industry. In this study, four hundred and twenty castrated male Ujimqin lambs which under the same feeding conditions were randomly selected as experimental samples. The A384T SNP was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the effect of this SNP on meat quality traits and fatty acid content were analyzed. The aim of this study was to investigate the association between the A384T SNP and meat quality traits in Ujimqin sheep. The results showed that there were 3 different genotypes (TT, TA and AA) at the A384T SNP site in Ujimqin sheep population. The frequency of TA genotype was the highest (0.4714), and the frequency of AA genotype was the lowest (0.1595) in this experimental animal population. The T allele was the dominant allele in this population. The genotype frequency of the A384T SNP was consistent with Hardy-Weinberg equilibrium (P>0.05). The pH₂₄ of individuals with TT genotype were significantly higher than those of TA and AA genotype (P<0.01). The drip loss of individuals with TT genotype were significantly higher than those of the TA and AA genotype (P<0.05). There were no significant differences among genotypes in other meat traits, including pH₁, carcass weight, net meat weight, eye muscle area and drip loss (P>0.05). The contents of palmitic acid and stearic acid in muscle tissue of TT genotype were significantly lower than those of TA and AA genotype individuals (P<0.01). The contents of myristic acid in muscle tissue of TT genotype were significantly lower than those of TA and AA genotype individuals (P<0.05). There were no significant differences in the other fatty acid contents among 3 genotypes (P>0.05). These data showed that the A384T SNP in the exon 3 of the LPL gene was associated with meat quality traits of Ujimqin sheep, and could be an effective genetic marker for molecular breeding of Ujimqin sheep. These results provide scientific basis for genetic improvement of Ujimqin sheep.

Key words： Ujimqin sheep Lipoprotein lipase gene (LPL) PCR-RFLP Fatty acid

Received: 10 September 2018

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Corresponding Authors: * zhangxiuying000@126.com

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	GAO Hong-Meng
	ZHANG Xiu-Ying
	ZHANG Hui-Ze
	HASI Qimuge
	ZHOU Xu-Guang
	WEI Jian-Min
	YU Zhong-Zheng

Cite this article:

GAO Hong-Meng,ZHANG Xiu-Ying,ZHANG Hui-Ze, et al. Association Analysis Between Polymorphism in the Exon 3 of LPL Gene and Meat Quality Traits in Ujimqin Sheep (Ovis aries)[J]. 农业生物技术学报, 2019, 27(3): 449-456.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.03.008 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I3/449

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