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Cloning of ZlGH3-8 Gene and Its Expression Analysis in Zizania latifolia During Development |
ZHAO Jin-Lan*, ZHOU Mei-Qi*, YE Zi-Hong, YAN Mu-Xi, ZHANG Ya-Fen, XIA Wen-Qiang, CUI Hai-Feng** |
Zhejiang Provincial Key Laboratory of Biometrology and Inspection / Quarantine College of Life Sciences, China Jiliang University, Hangzhou 310018, China |
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Abstract Pregnant Zizania latifolia is the result of stem organ expansion and development induced by Ustilago esculenta after infecting Zizania latifolia plants, but the regulatory mechanism of its expansion and development is still unclear. In this study, the full-length sequence of ZlGH3-8 (Zizania latifolia Gretchen Hagen 3-8) gene (GenBank No. MH355951) was cloned, the gene sequence was 1 916 bp in length and contained 2 introns. The cDNA was 1 174 bp in length, encoded 390 amino acids, which had a GH3 domain and a indoleacetic acid amide synthetase domain, and was highly similar to OsGH3-8 (GenBank No. XP_015647797.1) of Oryza sativa ssp. japonica. It was found that the expression change of ZlGH3-8 in plants was related to mycelial infection and proliferation of U. latifolia. The expression of ZlGH3-8 in stems was significantly higher than that in leaves (P<0.05). At the early stage of stem enlargement, the expression of ZlGH3-8 in gray Z. latifolia was significantly higher than that of white Z. latifolia, while the expression of male Z. latifolia was the lowest (P<0.05). Further analysis found that there were significant differences in the expression of ZlGH3-8 during the expansion and development of white Z. latifolia, indicating the ZlGH3-8 might be participated in the morphological development of stem organs of Z. latifolia, and it might be related to the down-regulation of immune defense response related to the infection of U. latifolia during pregnancy. This study preliminarily clarified the expression response of ZlGH3-8 in the development of Z. latifolia, which provides basic data for the study on the mechanism of expansion and development of Z. latifolia.
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Received: 27 March 2019
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Corresponding Authors:
hfcui@cjlu.edu.cn
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[1] 康璐瑶, 邢阿宝, 崔海峰, 等. 2017. ZlADR1基因的克隆及其在茭白孕茭中的表达[J]. 农业生物技术学报, 25(11): 1799-1808. (Kang L Y, Xing A B, Cui F H, et al.2017. Cloning and expression of ZlADR1 gene during the formation of swollen stem of Zizania latifolia[J]. Journal of Agricultural Biotechnology, 25(11): 1799-1808.) [2] 江解增, 邱届娟, 韩秀芹, 等. 2004. 茭白生育过程中地上各部位内源激素的含量变化[J]. 武汉植物学研究, 22(3): 245-250. (Jing J Z, Qiu J J, Han X Q, et al .2004. Changes of endogenous hormone contents of different parts during development of water bamboo (Zizania latifolia)[J]. Journal of Wuhan Botanical Research, 22(3): 245-250.) [3] 金晔. 2015. 茭白茎部膨大前后应激响应的蛋白质组分析[D]. 硕士学位论文, 中国计量学院, 导师: 叶子弘, pp. 49-50. (Jin Y.2015. Proteomic analysis of stress response of water shoot before and after bulking[D]. Thesis for M.S., China Jiliang University, Supervisor: Ye Z H. pp. 49-50.) [4] 李帅. 2016. 茭白Zl-RPM1和Zl-ADR1抗病基因的克隆及表达分析[D]. 硕士学位论文, 中国计量大学, 导师: 叶子弘, pp. 1-2. (Li S.2016. Cloning and expression analysis of Zl-RPM1 and Zl-ADR1 resistance genes in Zizania latifolia[D]. Thesis for M.S., China Jiliang University, Suppervisor: Ye Z H, pp. 1-2.) [5] 应荣, 崔海峰, 倪方群, 等. 2014 . 杀菌剂敌磺钠及植物生长调节剂对茭白孕茭的影响[J]. 植物生理学报, 50(7): 946-952. (Ying R, Cui H F, Ni F Q, et al. 2014. Effects of fungicide fenaminosulf and plant growth regulator on gall formation of Zizania latifolia[J]. Plant Physiology Journal 50(7): 946-952.) [6] 邹锋康, 王秋红, 周建朝, 等. 2018. 生长素调节植物生长发育的研究进展[J]. 中国农学通报, 34(24): 34-40. (Zou F K, Wang Q H, Zhou J C, et al.2018. Auxin regulating plant growth and development: Research progress[J]. Chinese Agricultural Science Bulletin, 34(24): 34-40.) [7] 周美琪, 李帅, 崔海峰, 等. 2018.菰ZlWRKY72转录因子克隆及其在茎部膨大发育中的表达分析[J]. 农业生物技术学报, 26(06): 920-930. (Zhou M Q, Li S, Cui H F, et al.2018. Cloning of ZlWRKY72 transcription factor and its expression analysis during the development of swollen stem of Zizania latifolia[J]. Journal of Agricultural Biotechnology, 26(06): 920-930.) [8] Agrios G N.eds. 1988. Plant Pathology[M]. Academic Press:5 edition, London, pp. 787-803. [9] Chan Y S, Thrower L B.1980. The host-parasite relationship between Zizania caduciflora Turcz. and Ustilago esculenta P. Henn. Ⅲ. Carbohydrate metabolism of U. esculenta and the host-parasite combination[J]. New Phytologist, 85(2): 217-224. [10] Ding X H, Cao Y L, Huang L L, et al.2008. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate- and jasmonateindependent basal immunity in rice[J]. The Plant Cell, 20(1): 228-240. [11] Emanuela P, Simon J U, Riccardo D M, et al.2019,Cytokinin-dependent control of GH3 group Ⅱ family genes in the Arabidopsis root[J]. Plant, 8(4): 94. [12] Feng S G, Yue R Q, Shen C J, et al.2015. Genome‐wide identification, expression analysis of auxin‐responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses[J]. Journal of Integrative Plant Biology, 57(9): 785-795. [13] Guo H B, Li S M, Peng J, et al.2007. Zizania latifolia Turcz. cultivated in China[J]. Genetic Resources & Crop Evolution, 154(6): 1211-1217. [14] Guilfoyle T J (1999) Auxin-regulated genes and promoters[C]//, Hooykaas P J J, Hall M A, Libbenga K R (eds). Biochemistry and Molecular Biology of Plant Hormones. Elsevier, Amsterdam, Netherland, pp 423-459 [15] Hagen G, Guilfoyle T J.1985. Rapid induction of selective transcription by auxins[J]. Molecular & Cellular Biology, 5(6): 1197-1203. [16] Hui S G, Hao M Y, Wang S P.2019. The groupⅠ GH3 family genes encoding JA-Ile synthetase act as positive regulator in the resistance of rice to Xanthomonas oryzae pv.oryzae[J]. Biochemical and Biophysical Research Communications, 508(4): 1062-1066. [17] Hsieh H L, Okamoto H, Wang M, et al.2000. FIN219, an auxin-regulated gene, defines a link between phytochrome A and the downstream regulator COP1 in light control of Arabidopsis development[J]. Genes & Development, 14(15): 1958-1970. [18] Jagadeeswaran G, Rainal S, Acharyal B R, et al.2007. Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae[J]. The Plant Journal, 51(2): 234-246. [19] Jain M, Kaur N, Tyagi A K.2006. The auxin-responsive GH3 gene family in rice (Oryza sativa)[J]. Functional and Integrative Genomics, 6(1): 36-46. [20] Khan S, Stone J M.2007. Arabidopsis thaliana GH3.9 influences primary root growth[J]. Planta, 226(1): 21-34. [21] Kong W L, Zhong H,Deng X X, et al.2019. Evolutionary analysis of GH3 genes in six Oryza species/subspecies and their expression under salinity stress in Oryza sativa ssp. japonica[J]. Plants, 8(2): 30. [22] Leyser O.2002. Molecular genetic of auxin signaling[J]. Annual Review of Plant Biology, 53(53): 377. [23] Liu Q, You W Y, Yu X P, et al.2011. A preliminary study on proteome variations associated with gall formation in Zizania latifolia trucs[J]. Plant Molecular Biology Reporter, 29(2): 360-368. [24] Navarro C, Villar I,Izuel-Rami M, et al.2006. FV20 Linezolld-associated peripheral neuropathy[J]. Basic & Clinical Pharmacology & Toxicology, 99(1): 41-50. [25] Navarro L, Zipfel C, Rowland O, et al.2004. The transcriptional innate immune response to fi922. Interplay and overlap with avrgene-dependent defense responses and bacterial pathogenesis[J]. Plant Physiolgy, 135: 1113-1128. [26] Staswick P E,Tiryaki I.2004.The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis[J]. The Plant Cell, 16(8): 2117-2127. [27] Staswick P E, Serban B, Rowe M, et al.2005. Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-aceticacid[J]. The Plant Cell, 17(2): 616-627. [28] Riemann M, Riemann M,Takano M.2008. Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling[J]. Plant, Cell and Environment, 31(6):783-792. [29] Robinson C J, Sailendra G, Narayan C T.2016. Investigation on the biotrophic interaction of Ustilago esculenta on Zizania latifolia found in the Indo-Burma biodiversity hotspot[J]. Microbial Pathogenesis, 98: 6-15. [30] Westfall C S, Sherp A M, Jez J M.2016. Arabidopsis thaliana GH3.5 acyl acid amido synthetase mediates metabolic crosstalk in auxin and salicylic acid homeostasis[J]. Proceedings of the National Academy of Sciences of the USA. 113(48): 13917-13922. [31] Willemsen V, Scheres B.2004. Mechanisms of pattern formation in plant embryogenesis[J]. Annual Review of Genetics, 38(38): 587. [32] Ye Z H, Pan Y, Zhang Y F, et al.2017. Comparative whole-genome analysis reveals artificial selection effects on Ustilago esculenta genome[J]. DNA Research, 24(6): 635-648. [33] Zhang Z Q, Li O, Li ZM, et al.2007. Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis Pseudomonas syringae interaction[J]. Plant Physiol, 145: 450-464. [34] Zhang J Z, Chu F Q, Guo D P, et al.2012. Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia[J]. Mycological Progress,11(2): 499-508. |
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