山药赤霉素受体基因<em>DoGID1A</em>的克隆及表达分析

doi:10.3969/j.issn.1674-7968.2019.11.004

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (5882 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Gibberellin receptor GID1 (gibberellin insentive dwarf 1), one of important elements in gibberellin (GA) signal transduction pathway, directly affects the effect of gibberellin on plant. A cDNA sequence encoding gibberellin receptor GID1 was cloned from epidermis of bulbils of 'Niuwei Yam' (Dioscorea opposita) with reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques. The expression of the cloned yam GID1 gene at different stages after bulbils were harvested and the response of the gene expression to paclobutrazole were tested by qRT-PCR. The results showed that the obtained gene had a full length of 1 123 bp with 996 bp opening reading fram encoding 331 amino acids, and named DoGID1A (GenBank No.MK268684). Bioinformation analysis showed that the molecular weight and theoretic isoelectric piont of the protein was 3.72×10⁴ D and 8.25, respectively. The DoGID1A protein was unstable protein with an abhydrolase domain (93~304 aa) but without transmembrance domain or signal peptide. Homologous alignment showed that DoGID1A protein shared 73.56% sequence similarity with 13 specieces including Musa acuminata, and shared conserved many domains including motifs linking hormone GA and protein DELLA. Phylogenetic tree showed all dicotyledons were cluster in a group, monocotyledonous plants except rice (Oryza sativa) and yam shared another group. Yam was clustered out alone, so was rice. qRT-PCR analysis showed the expression of DoGID1A gene increased during yam bulbil sprouting and was enhanced by paclobutrazol treatment. These results indicated that DoGID1A gene could be associated with yam bulbil sprouting. This study provides basic information for further research on expression pattern and function of this gene.

Received: 26 April 2019

ZTFLH:

S632.1

Corresponding Authors: xushengsheng@163.com

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LONG Wen-Hong
	MENG Jin-Gui
	XU Sheng-Sheng
	ZHANG Xue-Mei
	DUAN Yan-Bi
	YANG Rong-Ping

Cite this article:

LONG Wen-Hong,MENG Jin-Gui,XU Sheng-Sheng, et al. Cloning and Expression Analysis of Gibberellin Receptor Gene DoGID1A in Dioscorea opposita[J]. 农业生物技术学报, 2019, 27(11): 1933-1941.

URL:

http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.11.004 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I11/1933

[1] 陈敏, 马琳, 贾聪俊, 等. 2016. 紫花苜蓿赤霉素受体基因的克隆及表达分析[J]. 西北植物学报, 36(11): 2159-2166.
(Chen M, Ma L, Jia C J, et al.2016. Cloning and expression analysis of gibberellin receptor gene in Medicago sativa L.[J]. Acta Botanica Boreali-Occidentalia Sinica, 36(11): 2159-2166.)
[2] 董静, 尹梦回, 杨帆, 等. 2009. 棉花赤霉素不敏感矮化GID1同源基因的克隆和表达分析[J]. 作物学报, 35(10): 1822-1830.
(Dong J, Yin M H, Yang F, et al.2009. Cloning and expression profiling of gibberellin insensitive dwarf GID1 homologous genes from cotton[J]. Acta Agronomica Sinica, 35(10): 1822-1830.)
[3] 葛晖, 赵晓, 王增, 等. 2011. 葡萄GA受体基因 GID1初探及其在果实GA处理条件下的差异表达[J].中国农业大学学报, 16(2): 58-63.
(Ge H, Zhao X, Wang Z, et al.2011. Putative GA receptor gene family of Vitis vinifera L. and their differential expression under GA treatment[J]. Journal of China Agricultural Unive rsity, 16(2):58-63.)
[4] 刘芳, 袁华招, 沈欣杰, 等. 2013. 外源GA₃和PP₃₃₃对甜樱桃新梢生长及赤霉素代谢关键基因表达的影响[J]. 核农学报, 27(3): 0272-0278.
(Liu F, Yuan H Z, Shen X J, et al.2013. Effects of GA₃ and PP₃₃₃ on shoot growth and gene expression of gibberellins metabolism in Przfnus avium[J]. Journal of Nuclear Agricultural Sciences, 27(3): 0272-0278.)
[5] 龙雯虹, 郭华春, 肖关丽, 等. 2011a. 山药珠芽生长过程中激素和糖类物质含量的变化[J]. 园艺学报, 38(4): 753-760.
(Long W H, Guo H C, Xiao G L, et al.2011a.Variation of endogenous hormone and carbohydrate contents in growing yam bulbils[J]. Acta Horticulturae Sinica, 38(4): 753-760.)
[6] 龙雯虹, 王琼, 肖关丽, 等. 2013. 山药珠芽休眠期内源激素含量的变化及多效唑破除休眠的效应[J]. 西南农业学报, 26(5): 1996-2000.
(Long W H, Wang Q, Xiao G L, et al.2013. Variation of endogenous hormones in dormant yam bulbils and response of dormant bulbil to PP₃₃₃[J]. South-west China Journal of Agricultural Sciences, 26(5): 1996-2000)
[7] 龙雯虹, 肖关丽, 王琼, 等. 2011b. 多效唑和矮壮素破除山药零余子休眠的效应[J]. 中国蔬菜, (12): 56-59.
(Long W H, Xiao G L, Wang Q, et al. 2011b. Effect of paclobutrazol and chlormequat on dormancy-break of yam bulbils[J]. China Vegetables, (12): 56-59.)
[8] 罗弦, 陆涵, 袁雷, 等. 2016. 唐菖蒲赤霉素受体基因克隆及表达分析[J]. 西北植物学报, 36(11): 2152-2158.
(Luo X, Lu H, Yuan L, et al.2016. Cloning and experession analysis of gibberelin rceptor gene in Gladiolus hybridus[J]. Acta Botanica Boreali-Occidentalia Sinica, 36(11): 2152-2158.)
[9] 彭竹君, 田航, 王忆, 等. 2014. 不同时期叶面喷施多效唑对桃成花及FT基因表达的影响[J]. 中国农业大学学报, 19(3): 88-94.
(Peng Z J, Tian H, Wang Y, et al.2014. Impact of PP₃₃₃ foliar spays on FT gene expression and flower fud formation in peach[J]. Journal of China Agricultural University, 19(3): 88-94.)
[10] 岳川, 曾建明, 曹红利, 等. 2013. 茶树赤霉素受体基因CsGID1a的克隆与表达分析[J]. 作物学报, 39(4): 599-608.
(Yue C, Zeng J M, Cao H L, et al.2013. Cloning and expression analysis of gibberelin receptor gene CsGID1a in tea plant (Camellia sinensis)[J]. Acta Agronomica Sinica, 39(4): 599-608.)
[11] 岳建华, 张荻, 申晓辉. 2017. 百子莲赤霉素受体基因ApGID1全长的克隆及功能分析[J]. 上海交通大学学报(农业科学版), 35(1): 1-8.
(Yue E J H, Zhang D, Shen X H.2017. Cloning and functional analysis of the full length gibberelin receoptor gene ApGID1 from Agapanthus praecox ssp. Orientalis[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 35(1): 1-8.)
[12] Ariizumi T, Murase K, Sun T P, et al.2008. Proteolysis-independent down-regulation of DELLA repression by the gibberellin receptor GID1[J]. The Plant Cell, 20(9): 2447-2459
[13] Finkelstein R, Reeves W, Ariizumi T, et al.2008. Melecular aspects of seed dormancy[J]. Annual Review of Plant Biology, 59(1): 387-415.
[14] Griffiths J, Murase K, Rieu I, et al.2006. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis[J]. The Plant Cell, 18: 3399-3414.
[15] Gubler F, Millar A, Jacobsen J.2005. Dormancy release, ABA and pre-harvest sprouting[J]. Current Opinion in Plant Biology, 8(2): 183-187.
[16] Hirano K, Ueguchi-tanaka M, Matsuoka M.2008. GID1-mediated gibberelin sgnaling in plants[J]. Trends Plant Science, 13(4): 192-201.
[17] Kucera B, Cohn M A, Leubner-metzger G.2005. Plant hormone interaction during seed dormancy release and germintion[J]. Seed Science Reserch, 15: 281-307.
[18] Murase K, Hirano Y, Sun T P, et al.2008. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1[J]. Nature, 456: 459-463.
[19] Nakajima M, Shimada A, Takashi Y, et al.2006. Identification and characterization of Arabidopsis gibberelins receptors[J]. Plant Journal, 46: 880-889.
[20] Shimada A, Ueguchi-tanaka M, Nakatsu T, et al.2008. Structural basis for gibberellin recognition by its receptor GID1[J]. Nature, 456: 520-523.
[21] Sun T P.2010. Gibberellin-GID1-DELLA: A pivotal regulatory module for plant growth and development[J]. American Society of Plant Biologists, 154: 567-570.
[22] Ueguchi-tanaka M, Ashikari M, Nakajima M, et al.2005. GIBBERELIN INSINTIVE DWARF1 encodes a soluble receptor for gibberelin[J]. Nature, 437: 693-698.
[23] Yamamoto Y, Hirai T, Yamamoto E, et al.2010. A rice gid1 suppressor mutant reveals that gibberellin is not always required for interaction between its receptor, GID1, and DELLA proteins[J]. The Plant Cell, 11: 3589-3602.