|
|
|
| Variation of Pollen and Stigma Viability, Effect of Parental Chromosomal Numbers on Hybridization Between Different Populations of Pinellia ternata |
|
|
|
|
Abstract Abstract Hybridization is a critical pathway to obtain excellent germplasm, however, little publications are referred to hybridization of Pinellia ternata that is an important medicinal plant in China. In this study, different staining methods (iodine-potassium iodide (I-KI), peroxidase, triphenyl tetrazolium chloride (TTC), or acetocarmine method) and in vitro pollen germination were used to detect pollen viability; And benzidine-hydrogen peroxide method and artificial pollination were carried out to test stigma viability; Artificial pollination for different parental combinations that are originated 4 populations of P. ternata were executed to obtain hybrid seeds and traditional chromosome tabletting technique was employed to determine chromosome numbers for parents or hybrids. Results showed that the TTC staining was the most appropriate method to detect pollen viability; Pollen viability was maximum at 9:00~10:00 am in 2~3 day after flowering, Stigma possess high receptivity during 1 d before flowering to 2 d after flowering. The optimal temperature for pollen storage is 4 degree Celsius. Rates of hybrid seed setting (individuals or flower) for Hezhang×Baoding, Hezhang×Pingliang and Xiangyang×Hezhang were 43.75%, 11.11% and 33.33%, respectively whereas rates of hybrid seed setting (ovules) for these 3 combinations were 66.14%, 48.89% and 38.29% respectively. The chromosome number of all hybrids were consistent with its the female parent. These results indicated that new germpalsm of P. ternata could be obtained through hybridization between populations that possess different features. The present study further perfected technical link of hybridization breeding operation of P. ternata, which can be used to guide the practical operation of hybridization breeding of P. ternata.
|
|
Received: 04 October 2017
Published: 21 May 2018
|
|
|
|
| [1] 国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社, 2015:135.[2] 赵田生.半夏丰产栽培技术[J]. 农业科技与信息, 2000, (11):30.[3] 吴通兴, 叶隆平. 林地套种半夏栽培技术[J]. 浙江农业学报, 2004, (5):293[4] 谢杭.半夏一年三熟栽培及加工技术[J]. 江西农业经济, 2000, (4):52-53.[5] 靳光乾, 刘善新, 董宜广.半夏的林下种植技术[J]. 山东林业科技, 2004, (5):35-36.[6] 徐云碧.分子植物育种[S].科学出版社，2014.[7]WANG Y, XUE Y, LI J.Towards molecular breeding and improvement of rice in China[J].Trends Plant Sci., 2005, 10(12):610-614[8]杨成民, 魏建和, 隋春, 卢善发, 赵立子, 褚庆龙.我国中药材新品种选育进展与建议[J].中国现代中药, 2013, 15(9):727-737[9] 潘春香, 白音, 包英华, 范美玲.药用石斛种间有性杂交育种阶段性初报[J]. 时珍国医国药, 2010, 21(5), 1214-1215.[10]王秋颖, 郭顺星.天麻优良品种选育的初步研究[J].中国中药杂志, 2001, 26(11):22-24[11]姚入宇, 陈兴福, 张宝林, 李志飞, 杨兴旺.我国柴胡的种质资源现状与育种研究展望[J].中草药, 2013, 44(10):1349-1353[12]王康才, 肖亚雯, 罗庆云, 胡龙娇.半夏花粉活力检测及杂交研究[J].江西农业学报, 2012, 24(3):53-55[13] 林先明，张 军，谢玲玲, 郭杰一，刘海华，刘政华，唐春梓，甘国菊.半夏新品种鄂半夏2号的选育[J]. 湖北农业科学, 2014, 11(49).[14]魏莉霞，王国祥，彭云霞，蔡子平，王宏霞，李玉萍.半夏新品系BY-1的选育[J].北方园艺, 2014, (05):150-15[15]顾德兴, 李云香, 徐炳声.半夏的繁殖生物学研究[J].植物资源与环境学报, 1994, 3(4):44-48[16]李西文, 张晓柠, 刘海涛, 马小军, 陈震, 赵鑫.半夏有性繁殖研究[J].现代中药研究与实践, 2006, 20(5):59-61[17]贾学思, 王玉庆.半夏自交与杂交研究[J].中国农学通报, 2010, 26(7):195-198[18]侯典云, 王荔, 杨艳琼, 文国松, 杨凯, 黄平.半夏不同居群的细胞学研究[J].云南农业大学学报, 2005, 20(2):159-162[19]李名旺, 顾德兴, 刘友良.半夏属的染色体数目倍性与珠芽发生的关系[J].植物分类学报, 1997, 35(3):208-214[20]钟国成, 张利, 杨瑞武, 丁春邦.丹参及其近缘种花粉活力与柱头可授性研究[J].中国中药杂志, 2010, 35(6):686-689[21]张妍, 杨发君, 田义新.穿龙薯蓣花粉活力及柱头可授性测定方法研究[J].安徽农业科学, 2010, 38(5):2344-2346[22]王永周, 古松, 江莎.臭椿花粉生活力的测定方法及对比实验[J].内蒙古农业大学学报自然科学版, 2008, 29(1):88-92[23]罗长维, 李昆, 陈友, 孙永玉, 杨文云.膏桐花粉活力与柱头可授性及其生殖特性研究[J].西北植物学报, 2007, 27(10):1994-2001[24]刘林德, 张洪军, 祝宁, 申家恒.刺五加花粉活力和柱头可授性的研究[J].植物研究, 2001, 21(3):375-379[25]俞海琪, 寿伟国, 金永昌.半夏花器管特性及提高结实率研究[J].中国现代应用药学, 1991, 8(4):5-8[26]韩怡, 巢建国, 谷巍, 张莹, 孙亚昕.太子参花粉活力和柱头可授性研究[J].种子, 2012, 31(9):37-40[27] 崔艳芬, 杨明，伍菊仙, 胡学礼, 许艳萍, 曾民, 大麻花粉萌发的影响因子及花粉储存的初步研究[J].中国麻业科学, 2008，30（06）：305-310.[28]张连学, 常维春, 魏云洁, 柳丽, 王英平, 钱树文.人参、西洋参花药超低温保存对花粉存活的影响[J].植物学通报, 1993, 10(2):58-59[29]孙道旺, 马继琼, 尹桂芳, 蒋晓云, 陈燕萍, 徐云, 王玲.云南魔芋资源种间杂交育种初步研究[J].西南农业学报, 2013, 26(5):1992-1995[30] 张晓艳，覃章铮.半夏胚胎发育的研究[J]. 武汉植物学研究，1996，14(1)30-32 |
|
|
|
