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| Cloning and Expression Analysis of Tmem9B Gene in Different Chicken(Gallus gallus) Breeds |
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Abstract TMEM9B (transmembrane protein 9 domain family, member B), found in recent years, is a multifunctional transmembrane glycoprotein. Several studies have shown that TMEM9B protein plays key roles in multiple signaling pathways. However, the studies on the expression and functional properties of Tmem9B gene are seldom reported. In this study, Hy-Line brown chicken (Gallus gallus), Arbor acres plus broiler (AA+ broiler) and Sanhuang broiler were selected. The full-length coding sequences of Tmem9B gene were cloned from different chicken breeds and analyzed using bioinformatics tools. The expressional changes of Tmem9B gene in three breeds of normal chicken (AA+ broiler, Sanhuang broiler and Hy-Line brown chicken) and Escherichia coli lipopolysaccharide(LPS)-induced chicken inflammation model (AA+ broiler and Hy-Line brown chicken) were determined respectively to study the expressional activities and functional characteristics using quantitative real time polymerase chain reaction (qRT-PCR) technique. The results showed that there was only one SNP (single nucleotide polymorphism) site in the coding sequence of Sanhuang broiler Tmem9B gene, which meant that the gene was highly conserved among different chicken breeds. Tissue expression profile analysis showed that Tmem9B gene was all expressed in various tissues of AA+ broiler, Sanhuang broiler and Hy-Line brown chicken, but there were a little differences between different chicken breeds. Interestingly, the Tmem9B gene had high transcriptional activity in brain of all the three breeds. The expressions of Tmem9B gene of immune-related tissues were significantly up-regulated in the inflammation model chicken induced by LPS (lipopolysaccharide). This study showed hat Tmem9B may be a multifunctional gene that played important roles in many chicken biological processes, such as growth, development and inflammatory response. This study may provide theoretical basis for further explanation of the function and characteristics of Tmem9B gene.
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Received: 10 July 2017
Published: 04 February 2018
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