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| Detection of Six Kinds of Genetically Modified Soybean (Glycine max) by LAMP Method |
| , , , , ,ying chen |
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Abstract Abstract With the increasing of the global commercial production of genetically modified products, the detection of transgenic lines has gradually become the development trend of the detection of genetically modified components in internal and international. To carry out the regulations of genetically modified organisms in China's inward and outward, environmental release and labeling, and achieve effective management of genetically modified soybean (Glycine max), the loop-mediated isothermal amplification (LAMP) primers were designed according to the flanking sequence of genetically modified soybean DAS-81419-2, DP356043-5, A2704-12, FG72, BPS-CV127-9 and MON89788, separately. Through specificity, sensitivity and limit of detection testing, an LAMP method for the inspection of genetically modified soybean DAS-81419-2, DP356043-5, A2704-12, FG72, BPS-CV127-9 and MON89788 was developed. The results showed that this method could detect genetically modified soybean DAS-81419-2, DP356043-5, A2704-12, FG72, BPS-CV127-9 and MON89788 specifically and stably with a low detection limit of 0.1% (W/W). Since the LAMP detection could be finished within 30 min and the results could be directly detected according to the color change produced by SYBR Green Ⅰ dye with the naked eye. This method allowed rapid and accurate detection of genetically modified products on the inspection agency and relevant law enforcement departments, and non-essential of complex and expensive equipment.
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Received: 03 August 2016
Published: 13 January 2017
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Corresponding Authors:
ying chen
E-mail: chenyingcaiq@163.com
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