|
|
Preparation of Sheep (Ovis aries) and Cow (Bos taurus) Plancenta Immumoregulating Factor and Its Effects on Proliferation and Migration of PANC-Ⅰ Cells |
1, 1, 1, 1, 1, |
|
|
Abstract Placenta immunoregulating factor (PF) is composed of small polypeptides, which is extracted from the placenta. PF is valuable candidate for treatment of viral disease, immune deficiency disorder and malignant tumor. To detect the effects of sheep (Ovis aries) and cow (Bos taurus) placenta immunoregulating factor on the proliferation and migration of the human(Homo sapiens) pancreatic cancer cells-Ⅰ(PANC-Ⅰ) in vitro. PF was prepared from placenta by bienzyme hydrolysis. PANC-Ⅰ cells cultured in vitro were exposed to PF at the mass concentrations of 100~2 000 ng/mL. The effect of PF on the PANC-Ⅰ cell cycle was detected by flow cytometric method. The migration ability of PANC-Ⅰ cell was investigated with Transwell assay. The inhibition to proliferation rate of PANC-Ⅰ cells were evaluated by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay. The mRNA expression levels of tumor suppressor gene (P53) and cyclin-dependent kinase inhibitor 1A (P21) were analyzed by using real-time PCR. The relative expression was determined by using the comparative 2-??Ct method. The results showed that 0.588 mg PF derived from 17.11 g sheep placenta and 0.312 mg PF derived from 0.616g cow placenta, and the quality ratio was 20 000∶1. The morphology of the PANC-Ⅰ cells was not affect by adding the PF. The cell inhibition rate of PANC-Ⅰ cells was from 9.75%~22.89% with different concentration of sheep PF and from -3.81%~23.56% with different concentration of cow PF. The most remarkable concentration of sheep PF and cow PF were 500 ng/mL and 1 000 ng/mL, respectively. At the same time, the PI and SPF of PANC-Ⅰcells was the lowest with 500 ng/mL of sheep PF, but the proliferation index (PI) and S-phase fraction (SPF) were not affected by cow PF. The migration ability of PANC-Ⅰcells was not affected by PF. The expression levels of P53 and P21 of PANC-Ⅰcells were increased and decreased respectively after the treatment of PF. The results showed that the PF had a negative effect on the proliferation of PANC-Ⅰ cells in vitro, and the different effects of sheep and cow PF on PANC-Ⅰcells may related to the composition of the PF. This study provides new ideas for the treatment of pancreatic cancer.
|
Received: 13 June 2016
Published: 07 November 2016
|
|
|
|
[1] 房新平,夏文水,生庆海,等. 论胎盘的功能及其开发应用[J]. 食品研究与开发,2006,27(9):189-190.[2] 牟德华,李艳,赵玉华. 牛胎盘生物活性物质的研究进展[J]. 河北工业科技,2006,23(2):120-123.[3] 许代娣,李琪. 胎盘免疫调节因子的研究现状[J].广西医科大学学报,1999,16(2):1-5.[4] 苏晔,魏泓.胎盘免疫调节因子的生物功能及临床应用进展[J].免疫学杂志,2002,18(3):117-119.[5] Jemal A,Siegel R,W ard E,et al.Cancer statistics,2007[J].CA Cancer J C lin,2007,57(1):43-66.[6] 刘海林,王磊.胰腺癌早期诊断与治疗的研究进展[J].世界华人消化杂志,2009,17(34):3475-3479.[7] 朱蓓薇,葛瑞宏. 双酶水解法制备羊胎盘活性肽[J].生产与科研经验,2003,29(11):41-45.[8] 李硕,赵晓云,常世杰,等. 酶解法和超滤法获得羊胎盘提取物对人脐静脉内皮细胞增殖影响差异[J]. 解剖科学进展, 2011,17(6):548-551. [9] Keim V. Role of genetic disorders in acute recurrent pancreatitis.World J Gastroenterol 2008;14:1011-1015[10] Whitcomb DC. Genetic aspects of pancreatitis.Annu Rev Med 2010;61:413-424[11] 覃柳燕,张学荣,等. 胎盘免疫调节因子对HepG 2.2.15细胞毒性及HBV DNA分泌作用的影响[J].山东医药,2010,50(3):16-17. [12] Angelucci,C,Lama G,Sica G.The growth of malignant and nonmalignant human cells is modulated by a human placental extract[J].Anticancer Res,1999,19(1A):429-436.[13] 张京田,丁晓红,李选社.强化胎盘因子对细胞因子产生的影响[J].西安医科大学学报,2007,16(3):238-240. [14] 丰慧根,李延兰,任太芳,等.胎盘免疫调节肽联合化疗药物抗肿瘤作用的研究[J].中国生化药物杂志,1999,20(3):129-130. |
|
|
|