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SNPs Analysis of LAMB1 Gene in Fine-wool Sheep (Ovis aries) Based on the DNA Pools and Re-sequencing Technologies |
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Abstract Laminin-beta-1 (LAMB1) gene is the member of the laminin family, and plays an important role in biological processes. To analyze the genetic effects associated with the wool traits of candidate genes LAMB1, based on DNA pools with the re-sequencing technology to screened 5 single nucleotide polymorphisms (SNPs) data of LAMB1 gene exon regions, combining with single strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) technology to type the genotype, the genetic effects of LAMB1 gene 5 SNPs on wool traits of totally 418 sheep (Ovis aries) in fine-wool sheep at Xinjiang Kunes farm were analyzed by the GLM of SAS 8.1. Linkage disequilibrium (LD) was used by HaploView 4.2. The results showed this population genetic variations of fine-wool sheep was in the medium level, there were 5 non-synonymous mutation SNPs. AA and GA genotype in the identification of the body weight and body weight after shearingk of the individual value was extremely significant higher than GG genotype on SNP2 (P<0.01). CC, TT and TC genotypes in the staple length of the individual value was extremely significant on SNP4(P<0.01). CC genotype in the body weight after shearingk of the individual value was extremely significant higher than TT and TC genotype on SNP4 (P<0.01). While, each genotypes in the part of wool traits on other SNPs were significance (P<0.05). SNP1 and SNP3, SNP2 and SNP3 were in the linkage disequilibrium state, LAMB1 gene might be used as one of a potential and valuable candidate molecular marker on wool traits in fine-wool sheep. The study revealed the molecular genetic features of LAMB1 gene and evaluated the genetic relationship in fine-wool sheep. The results provide molecular genetic basis for efficient breeding of fine-wool sheep breeds, and efficient conservation and utilization the germplasm resources.
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Received: 26 May 2016
Published: 07 November 2016
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