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| Associations of Calpastatin Gene polymorphism in the 5' Regulatory Region with Meat Quality Traits in Chicken (Gallus gallus) |
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Abstract Calpatatin (CAST) is the endogenous inhibitor of the calpain system, which has been found to affect the postmortem tenderization of skeletal muscle. This experiment was designed to study the effects of polymorphism of 5' flanking regulating of CAST gene on meat quality traits in broiler (Gallus gallus), F、D、Y、B and S3 5 strains of quality broiler lines were used in the present study. The genetic polymorphisms of calpastatin (CAST) was investigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP), and analyze the association of polymorphisms sites with quality broiler lines which included the PH value, Vitamin B1, muscle color, water holding capacity, intramuscular fat content and density of muscle fiber. In the present study, the 5' regulatory region of CAST gene of 300 broilers was amplified and a uniform fragment of 210 bp was obtained. The results indicated that one SNP site was detected in 5' flanking regulating of CAST gene. The 210 bp PCR product was digested with restriction endonuclease BMPI. Three genotypes (AA, AG and GG) were found at G-198A mutation locus at a distance of CDS-198 locus in 300 broilers by digesting the PCR product with BMPI, and three frequency of AA, AG and GG genotypes was 0.3, 0.2, 0.5, respectively in Y strain (0,125, 0.1, 0.775, respectively in S strain; 0.2, 0.375, 0.425, respectively in F strain; 0.05, 0.35, 0.6, respectively in D strain; 0.1897, 0.3966, 0.4138, respectively in B strain.). The fitness test showed that all quality broiler lines at three mutation locus were in Hardy-Weinberg equilibrium. The relationship between CAST gene and meat quality traits in quality broiler was analyzed by least squares linear model. The individuals with AA genotype had significant effect on the intramuscular fat content and density of muscle fiber(P＜0.05), and has no significant effect on the other meat quality traits(P＞0.05). AA was the most favorable genotype for meat quality traits in broiler. The results preliminarily indicate that allele A of G-198A polymorphic site of CAST gene is a potential DNA marker for improving meat quality traits in broiler. The information found in the present study is very important for improving meat quality in broiler by marker assisted selection.
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Received: 20 April 2015
Published: 23 November 2015
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