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| Molecular Cloning and Expression Analysis of GR Gene in Pigeon (Columba livia domestica) |
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Abstract Glucocorticoid receptor (GR) is a member of the conservative nuclear receptor superfamily. It belongs to nuclear transcription factor. It plays an important role in maintaining the stability of the body and animal breeding and metabolism. The aim of this experiment was to clone the cDNA full length of GR gene of pigeon (Columba livia domestica) and analyze its expression profile. The specific primers were designed based on the published necleotide sequence of chicken (Gallus gallus domesticus) . The sequencing results showed that GR gene containing 2 313 bp CDS, 102 bp 5'UTR and 18 bp 3'UTR, encoding a protein of 770 amino acids(GenBank accession No: NM_001037826.1). The sequence homology analysis showed that the pigeon GR gene shared 94%, 85% and 75% homology of the amino acid sequence with chicken, crocodlie (Alligator mississippienses) and human (Homo sapiens), respectively. The amino acid sequence analysis of Chicken, crocodile and human found the GR amino acid sequence had a very conserved DNA binding domain (DBD), and a higher conserved hormone recognition domain (LBD), in addition to a GR specific region. qRT-PCR results suggested that GR mRNA were expressed in the different organizations. And the expression quantity from high to low were testis, pancreas, lung, ovary, abdominal fat, liver, heart, skeletal muscle, kidney, spleen, oviduct, stomach, hypothalamus, brain, intestine and craw successively. The expression of GR gene in the testis was significantly higher than other tissues (P＜0.05), followed by the pancreas and lung (P＜0.05). In this study, we obtained the full length of GR gene cDNA and the expression of GR gene, this results provide a basic data for further study on the function of GR gene.
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Received: 07 August 2015
Published: 23 November 2015
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