Contact Us Add to Favorite
 
NianQi Search Adv Search
33
  2015, Vol. 23 Issue (8): 1067-1075    DOI:
Articles and Letters Current Issue | Archive | Adv Search |
Genome-wide Association Study on Market Weight Based on Specific-locus Amplified Fragment Sequencing (SLAF-seq) in Jinghai Yellow Chicken (Gallus gallus)
Download: PDF (3400 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  In order to reveal the genetic basis of market weight trait and scan molecular markers and candidate genes which can be used to improve the market trait, market weight of 400 female Jinghai yellow chicken (Gallus gallus) in core group was measured at 16 weeks of age. Genome-wide association study (GWAS) was carried out using specific-locus amplified fragment sequencing technology to detect nucleotide polymorphisms (SNPs) associated with market weight. Finally, 15 single SNPs that associated with market weight were detected using General liner model (GLM). Four SNPs reached 5% Bonferroni genome-wide significance (P<1.87E-06) and 11 SNPs reached “suggestive” genome-wide significance (P<3.73E-05). 6 SNPs that associated with market weight were detected using mixed linear model (MLM). 2 SNPs reached 5% Bonferroni genome-wide significance (P<1.87E-06) and 4 SNPs reached “suggestive” genome-wide significance (P<3.73E-05). 6 SNPs of rs1169337928, rs475641139, rs31633105, rs475856030, rs476878211 and rs477915563 were detected both using GLM and MLM. Genes in the candidate regions with 1-Mb windows (SNP position±0.5 Mb) surrounding each significant SNP were screened. Finally, 14 candidate genes were obtained, among which 6 genes of FAM124A, QDPR, BCL11A, LDB2, BOD1L1 and WDR1 might be important candidate genes influencing market weight. Furthermore, It was also found that 9 out of 14 SNPs were distributed in the region of 75.641~79.592 Mb on chromosome 4, and this region was important candidate region which influenced the market weight of Jinghai yellow chicken. This study provides theory material for marker assisted selection of Jinghai yellow chicken.
Key wordsSLAF-seq      Jinghai yellow chicken      Market weight      Genome-wide association study (GWAS)     
Received: 16 January 2015      Published: 01 June 2015
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
ZHANG Shou
YU Wen-Gao
ZHANG Xiang-Jian
ZHANG Gen-Xi
YU Jin-Yu
HAN Hun-Peng
YU Yong-Juan
Cite this article:   
ZHANG Shou,YU Wen-Gao,ZHANG Xiang-Jian, et al. Genome-wide Association Study on Market Weight Based on Specific-locus Amplified Fragment Sequencing (SLAF-seq) in Jinghai Yellow Chicken (Gallus gallus)[J]. , 2015, 23(8): 1067-1075.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I8/1067
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech