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Abstract Heterogeneous nuclear ribonucleoprotein K (hnRNP K), a member of hnRNP complex, can interact with nucleic acids and various proteins. The structure of hnRNP K protein mainly contains three K homology (KH) domains that can combine with DNA or RNA, and one K protein interactive region (KI) domain involved in protein interaction. It also contains a nuclear localization signal (NLS) in the N terminal that mediates its transport from the cytoplasm to the nucleus and a nuclear shuttling domain (KNS) in the C terminal that confers the ability to translocate bi-directionally through the nuclear pore complex. In eukaryotes, hnRNP K expresses in many type of cells and tissues, but the expression levels vary during different developmental phases, and it is located predominantly in the nucleus and cytoplasm, also in mitochondria and plasma membrane. In addition, hnRNP K is subject to several post-translational modifications, such as phosphorylation, methylation, sumoylation, ubiquitylatio, acetylation, and so on, which can regulate its interactions with different molecules and influence its functions. The complexity of structure, expression, location and the diversity of post-translational modifications in hnRNP K decide its abundant biological functions, which can participate in transcription, translation, RNA splicing, DNA repair, chromatin remodeling, and also have important roles in spermatogenesis, nervous system and ovary development, organogenesis and carcinogenesis process. Among them, spermatogenesis is tightly regulated and well-studied process consisting of a series of highly specific cellular processes and multiple genes involved in the final formation of the sperm. Many research have shown that hnRNP K may play a key role in spermatogenesis regulation by its special structure, selective splicing and expression pattern. This review focuses on the structure, expression regulation, post-translational modification and biological functions of hnRNP K, and its roles in spermatogenesis. It provides initial value?for?studying?the?biological?functions?of?hnRNP K especially in spermatogenesis.
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Received: 08 October 2014
Published: 17 March 2015
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