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| Identification and Analysis of Differential Expression Proteins Realated to Chilling Tolerance in Longjiao 2 (Zizania latifolia) |
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Abstract As a main aquatic vegetable in southern China, Zizania latifolia could be affected by temperature in the field. The two double-cropping cultivars of Z. latifolia were comparatively analyzed by the method of two-dimensional electrophoresis (2-DE), including the chilling tolerance cultivar of Longjiao 2 and the main cultivar of Zhejiao 911. Among the 822 to 955 protein spots of leaves, differential expression protein spots were selected and analyzed. It was found that the cultivar of Longjiao 2 contained more up-regulated spots and fewer down-regulated spots than that of Zhejiao 911 under the treatments of low temperate and recovery, respectively. Six differential expression chilling tolerance protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF-TOF MS), including IAA auxin amido hydrolase (IAA-AH), putative formate-tetra-hydrofolate ligase (FSH), malate dehydrogenase (MDH), mannose-6-phosphate reductase (M6PR), ATP synthase CF1 alpha chain (ATPase) and the molecular chaperon cpn60-1, which were related to the plant hormone metabolism, nucleic acid synthesis, energy metabolism, photosynthesis and protein folding, respectively. The expression trend of IAA-AH, MDH and the molecular chaperon of cpn60-1 were similar in the two cultivators of Longjiao 2 and Zhejiao 911 with increasing expression in the treatments of low temperature and decreasing expression in the following treatments of recovery, while the other three were expressed differently between the two cultivators. Especially in the treatments with the temperate of 5 degrees, there were more steady changes of expression and better recovery in the cultivator of Longjiao 2 than that in Zhejiao 911. Based on transcriptome sequencing of Z. latifolia, parts of the coding sequences of 6 differential expression proteins were cloned and the changes of expression were analyzed by Real-time PCR. In this research, the proteins related to chilling tolerant of Longjiao 2 were identified and discussed, which could be contributed to the illumination of the special features of Longjiao 2 and chilling tolerance. The results would provide theoretical basis to the breeding of Z. latifolia cultivars which could be harvested in different times.
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Received: 08 October 2014
Published: 01 March 2015
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