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| Identification and Expression Analysis of Heat Shock Factor (Hsf) Gene Family in Tomato (Solanum lycopersicum) |
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Abstract Heat shock factor (Hsf), a kind of transcription regulation gene family in plants, plays an important role in response to high temperature and other vital movement. In this study, Hsf were identified using tomato (Solanum lycopersicum) whole-genome sequencing results. Multiple sequence alignments were performed using the Cluster W and Bioedit program. A phylogenetic tree was created using the MEGA5 program. Chromosomes were located by MapDraw program. Expression pattern of Hsf gene family at different development stages and in response to high temperature were analyzed based on the existing microarray database and qRT-PCR. Results showed that the tomato Hsf gene family contained 25 members. Sequence alignment showed that these members had a DNA binding domain (DBD) of specific transcription factor. Transcription factors from Arabidopsis thaliana, rice(Oryza sativa) and cucumber(Cucumis sativus L.) were divided into three branches (A, B and C), and further subdivided into 15 categories (A1~A9, B1~B4 and C1~C2). Chromosomal localization found that the Hsf unevenly were distributed in the ten of twelve tomato chromosomes except Chr.1 and Chr.5. Most of the Hsf genes had differential expression pattern and response to high temperature at seedling stage. The results provide foundation for revealing function of Hsf genes in tomato.
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Received: 29 August 2014
Published: 01 March 2015
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