|
|
|
| Cloning and Expression Analysis of the Transcription Factor Gene (GhMYB11) in Gossypium hirsutum L. |
| 1, 1, 1, 1, 2 |
1.
2. Shandong Cotton Research Center
|
|
|
|
|
Abstract MYB gene family are important transcription factors involving in plant responses to biotic and abiotic stresses by controlling the expression of diverse functional genes. Utilizing genomic sequences of diploid Gossypium raimondii, a new cotton MYB transcription factor gene GhMYB11 (GenBank accession No. HQ234875.1) was cloned from upland cotton(G. hirsutum) cultivar Lumianyan 32. The full length of GhMYB11 cDNA was 1 001 bp coding an open reading frame (ORF) of 828 bp. There were 11 SNPs (single nucleotide polymorphism) between the ORF sequences of GhMYB11 and G. raimondii 001G177100.1, of which 7 SNPs caused amino acid alternation. Phylogenetic analysis showed that GhMYB11 was highly similar (E value=7e-83 and 1e-90, respectively) to AtMYB13/14, which were 2 important factors involving in ABA synthesis and signal transduction in Arabidopsis thaliana, with 2 highly conservative MYB DNA binding domains R2R3 and a transactivation domain. Real-time quantitative PCR analysis revealed that after Verticillium dahliae strain VD8 inoculated, GhMYB11 expression of cotton seedlings very significantly up-regulated by 14.49 times(P＜0.01) in cotton leaves and by 5~6 times significantly(P＜0.05) after 10% PEG6000, 150 mmol/L NaCl and 200 mmol/L H2O2 treated, respectively. The result suggested that GhMYB11 maight play a key role in cotton responding to biotic and abiotic stresses and could be treated as an important candidate gene in genetic improvement for stress resistance of upland cotton cultivars. This work provides basic materials for cotton stress tolerance enhancement through genetic engineering.
|
|
Received: 26 June 2014
Published: 13 January 2015
|
|
|
|
| 陈清, 汤浩茹, 董晓莉, 等. 2009. 植物Myb转录因子的研究进展[J]. 基因组学与应用生物学, 28(2): 365-372. (Chen Q, Tang H R, Dong X L, et al. 2009. Progress in the sudy of plant Myb transcription factors[J]. Genomics and Applied Biology, 28(2): 365-372.)丁振华, 李世明, 安学丽, 等. 2009. MYB15转基因表达增强拟南芥的脱落酸敏感性和抗旱性[J]. 遗传学报, 36(1): 17-29. (Ding Z H, Li S M, An X L, et al. 2009. Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana[J]. Journal of Genetics and Genomics, 36(1): 17-29.)刘任重. 2010. 湘杂棉2号杂种优势的遗传机理研究[D]. 博士学位论文, 南京农业大学, 导师: 张天真, 章元明, pp.108-110. (Liu R Z. 2010. Study on genetic mechanism of heterosis in hybrid cotton XZM2[D]. Dissertation for Ph.D., Nanjing Agricultural University, Supervisor: Zhang T Z and Zhang Y M, pp.108-110.)Abe H, Urao T, Ito T, et al. 2003. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling[J]. The Plant Cell Online, 2003, 15(1): 63 -78.Ascencio-Ibá?ez J T, Sozzani R, Lee T J, et al. 2008. Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection[J]. Plant Physiology, 148(1): 436-454.Cao D, Cheng H, Wu W, et al. 2006. Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis[J]. Plant physiology, 142(2): 509-525.Cedroni M L, Cronn R C, Adams K L, et al. 2003. Evolution and expression of MYB genes in diploid and polyploid cotton[J]. Plant Molecular Biology, 51(3): 313-325.Chen S C, Peng S Q, Huang G X, et al. 2003. Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in Hevea brasiliensis[J]. Plant Molecular Biology, 51(1): 51-58.Chen Y H, Yang X Y, He K, et al. 2006. The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family[J]. Plant Molecular Biology, 60 (1): 107-124.Cominelli E, Galbiati M, Vavasseur A, et al. 2005. A guard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance[J]. Current Biology, 15(13): 1196-1200.Dai X, Xu Y, Ma Q. 2007. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis[J]. Plant physiology, 143(4): 1739-1751.Dat J, Vandenabeele S, Vranová E, et al. 2000. Dual action of the active oxygen species during plant stress responses[J]. Cellular and Molecular Life Sciences, 57(5): 779-795.Denekamp M, Smeekens S C. 2003. Integration of wounding and osmotic stress signals determines the expression of the AtMYB102 transcription factor gene[J]. Plant Physiology, 132(3): 1415-1423.Dubos C, Stracke R, Grotewold E, et al. 2010. MYB transcription factors in Arabidopsis[J]. Trends in plant science, 15(10): 573-581.Jung C, Seo J S, Han S W, et al. 2008. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis[J]. Plant physiology, 146(2): 623-635.Knight H, Knight M R. 2001. Abiotic stress signalling pathways: specificity and cross-talk[J]. Trends in plant science, 6(6): 262-267.Liang Y K, Dubos C, Dodd I C, et al. 2005. AtMYB61, an R2R3-MYB transcription factor controlling stomatal aperture in Arabidopsis thaliana[J]. Current Biology, 15(13): 1201-1206.Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using realtime quantitative PCR and the 2-ΔΔCT method[J]. Methods, 25(4): 402-408.Loguercio L L, Zhang J Q, Wilkins T A. 1999. Differential regulation of six novel MYB-domain genes defines two distinct expression patterns in allotetraploid cotton (Gossypium hirsutum L.) [J]. Molecular Genetics and Genomics, 261(4-5): 660-671.Machado A, Wu Y, Yang Y, et al. 2009. The MYB transcription factor GhMYB25 regulates early fibre and trichome development[J]. Plant Journal, 59(1): 52-62.Ma Q, Dai X, Xu Y, et al. 2009. Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes[J]. Plant Physiology, 150(1): 244-256.Paz-Ares J, Ghosal D, Wienand U, et al. 1987. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators[J]. The EMBO Journal, 6(12): 3553-3558.Pu L, Li Q, Fan X, et al. 2008. The R2R3 MYB transcription factor GhMYB109 is required for cotton fiber development[J]. Genetics, 180 (2): 811-820.Rabinowicz P D, Braun E L, Wolfe A D, et al. 1999. Maize R2R3-Myb genes: sequence analysis reveals amplification in the higher plants[J]. Genetics, 153(1): 427-444.Riechmann J L, Heard J, Martin G, et al. 2000. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes[J]. Science, 290(5499): 2105-2110.Seo P J, Xiang F, Qiao M, et al. 2009. The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis[J]. Plant Physiology, 151(1): 275-289.Stracke R, Werber M and Weisshaar B. 2001. The R2R3-MYB gene family in Arabidopsis thaliana[J]. Current opinion in plant biology, 4(5): 447-456.Suo J, Liang X, Pu L, et al. 2003. Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L)[J]. Biochimica et Biophysica Acta, 1630(1): 25-34.Vannini C, Locatelli F, Bracale M, et al. 2004. Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants[J]. The Plant Journal, 37(1): 115-127.Villalobos M A, Bartels D, Iturriaga G. 2004. Stress tolerance and glucose insensitive phenotypes in Arabidopsis overexpressing the CpMYB10 transcription factor gene[J]. Plant Physiology, 135(1): 309-324.Wang S, Wang J W, Yu N, et al. 2004. Control of plant trichome development by a cotton fiber MYB gene[J]. Plant Cell, 16(9): 2323-2334.Wu Y, Machado A C, White R G, et al. 2006. Expression profiling identifies genes expressed early during lint fibre initiation in cotton[J]. Plant Cell Physiology, 47(1): 107-127. |
|
|
|
