|
|
|
| PCR on Adulteration Detection of Pork, Beef and Mutton |
| 1, 1,韦露450103198306212562李丽婷120103198310162928 |
|
|
|
|
Abstract With more and more appearence of meat adulteration, one simple, high-effency and quick detection method is urgent which compares to tradional ones with expensive, low-accuracy and low-efficiency. In this study, adulteration detection with molecular biology techniques was researched. A pair of highly specific primers for pork, beef and sheep were obtained, respectively, by trational PCR screening and verification. The Real-time fluorescence quantitative PCR results of pork, beef and sheep with 3 primer combinations showed that the detection limits were 1.69 copy, 1.52 copy and 17.5 copy, respectively. The 3 primer combinations were then developed into detection kits for pork, beef and mutton. The kits developed in this study identified pork, beef and mutton effectively when they were used to detect several deep-processed meat products in market. The kit is simple, which shows a good application prospect in field use.
|
|
Received: 19 August 2013
|
|
Corresponding Authors:
韦露450103198306212562李丽婷120103198310162928
|
|
|
|
| [1] |
NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
|
|
|
|
