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Abstract Due to the characterization of infect a broad range of cell strains and high efficiency of integrate into genome of the cells, lentivirus vector has been widely used in preparation of transgenic animals. This research aims to produce a high level titer lentivirus with relatively lower cost, we constructed the lentivurus vector which contained the green fluorescent protein and red fluorescent protein, after transfect the three lentivirus vectors into the 293T cells with calcium phosphate transfection mathod, the transfection efficiency up to 72%, this method reduced the cost to produce lentivirus vector. We used high speed centrifugation and ultracentrifuge method to purify the lentivirus vector, after the purification and concentration we got the 80 μL purified lentivirus vector with the titer up to 2.38×109 TU/mL from 150 mL packaged culture medium. This lentivirus vector can be used for producting double foreign genes transgenic animals and comparing the effect of the two different report genes in screening of the transgenic offspring.
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Received: 20 September 2010
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