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Abstract Genetically modified maize with high lysine LY038 has been widely applied in animal feed, but so far there has been no report about the method of amplification for flanking sequence of LY038 and its event-specific qualitative PCR detection. In this study, the 5'- flanking sequence of integrated gene-construct of genetically modified maize LY038 was characterized by modified adapter-linked PCR (M-AL-PCR). According to the 5'- flanking sequence, the event-specific primers were designed，and the event-specific qualitative detection of genetically modified plant LY038 was established to produce a 175 bp product. With genetically modified maize (Zea mays L.) LY038, MIR604, Bt176, Bt11, MON810, MON863, GA21, NK603, non-genetically modified maize, genetically modified rice (Oryza sativa L.) Cry1C* and Cry2A*, genetically modified soybean (Glycine max) Roundup Ready and genetically modified oilseed rape (Brassica campestris L.) GT73 as materials, this method developed in this work proved to be highly specific and sensitive, and the limit of detection was 0.1%. It is suggested that the developed qualitative detection system can accurately, fast and efficiently identify the genetically modified maize LY038 and its derivates.
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Received: 01 November 2010
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