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| QTL Analysis Related to Feed Conversion Efficiency in Common Carp (Cyprinus carpio) Using SSR and EST Markers |
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Abstract One hundred and thirty seven SSR (simple sequence repeat) and EST（expressed sequence tag） markers were used to analyze the genotype of 93 individuals of BC1F1 generation which cross from Barbless carp (Cyprinus pellegrini) and Hebao-cold tolerance red carp(Cyprinus carpio wuyuanensis). Using Windows Map Manager 2.0 software analyzed the quantitative trait related to feed conversion efficiency by single marker regression analysis. Results showed that eight SSR markers ( HLJ133, HLJ360, HLJ380, HLJ562, HLJ740, HLJ852, HLJ930 and HLJ1420) and two EST markers ( HLJE318 and HLJE417 ) were discovered related to common crap's feed conversion ratio. The phenotype variation was explained by these loci, ranged from 4% to 11%. The genotypes of these correlative loci were determined by ANOVA analysis. In loci HLJ360、HLJ380 and HLJ562 respectively, genotypes 212/212, 236/224 and 220/220 are positive correlation to feed conversion efficiency trait. These functional markers and genotypes which related to common crap's feed conversion may be used for the QTL mapping and marker assisted selection in common carp.
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Received: 30 October 2009
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