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| Effects of Oxygen Tension, Medium and Well of the Well on In vitro Development of Mouse Embryo |
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Abstract The objectives of the present research are to investigate whether embryo culture media had prefere- nces to oxygen tension during the in vitro development of mouse early embryos, and to explore feasibility of using physical lung air to support the in vitro development of mouse embryos, and to evaluate effect of well of the well(WOW) culture on in vitro pre-implantational deveopment of mouse embryos. The results were: First, cleavage rate and blastocyst rate were not significantly different between medium CZB and mKSOM on the use of three gas phases: 4%CO2＋16%O2＋78%N2＋2%H2O (lung air), 5%CO2＋5%O2＋90%N2 (5%O2, low oxygen) and 5%CO2＋20%O2＋75%N2(20%O2, high oxygen) (P＞0.05), but mean total cell numbers per blastocyst cultured in CZB medium were higher than that in mKSOM when the lung air was used(P＜0.05). Second, based on mKSOM medium, the blastocyst rate(22.6%) in 5%O2 gas phase was notably higher than that in other two gas phase(P＜0.05). Third, as for CZB medium, blastocyst rate was not different significantly among three gas environments (P＞0.05). Fourth, both the blastocyst rate （74.6±5.1%） and the mean total cell numbers per blastocyst（76±2） cultured in WOW system were obviously higher than that in the group culture system(38.2±6.6% and 58±4). Taken together, these results indicate that the mKSOM medium and the CZB medium have their corresponding preferences to oxygen tension during in vitro culture of mouse embryos, and the lung air was reaffirmed to be able to effectively support in vitro pre-implantation development of mouse embryos, and WOW culture system can apparently enhance the in vitro developmental competence and blastocyst quality of mouse embryos.
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Received: 31 August 2009
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