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Abstract Abstract A number of promoter fragments from Panebacillus polymyxa M-1 were cloned using a promoter-trap vector, pECE7. Three promoter fragments of the highest chloramphenicol (Cm) resistance were selected through the concentration gradient of Cm, which were named p5, p8 and p17, respectively. A pair of primer was designed to obtain the gfpmut3a. Then the gfpmut3a and the promoter fragments were inserted into the Escherichia coli-Bacillus shuttle vector pHY300PLK to construct the vectors of pGFP5, pGFP8 and pGFP17. The new gfp vectors were transformed into E.coli DH5α by heat shock, and the bright fluorescence of E.coli-gfp5, E.coli-gfp8 and E.coli-gfp17 was observed by the confocal laser-scanning microscopy. Meanwhile the new vectors were respectively transformed into Bacillus cereus B905 by electroporation and the engineering bacteria with GFP, Bacillus cereus B905（gfp-5, gfp-8 and gfp-17） were obtained. The results clearly showed bright green fluorescence of the transformants, and the fluorescence of the B905-gfp17 was less than that of the others.
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Received: 12 March 2009
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Corresponding Authors:
Qi Wang
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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