Abstract Abstract In order to explore the role of vitamin A(VA) on fatty deposition and cholesterol cleaning, the distribution of hepatic lipid droplet in high-fat diet fed mouse(Mus musculus) was observed by paraffin section preparation methods; the relative level of hepatic stearolysis related gene mRNA was determined by real-time RT-PCR and serum cholesterol(CHO) and high density lipoprotein-cholesterol(HDL-C） levels. Results showed that: first three days there was no significant difference between VA treated and control group on the daily gain(P>0.05); on the 4th, 9th and 27th day the daily gain was significantly lower than that of control group (P<0.05); the subcutaneous fat and fat pad under peritoneum were significantly lower in VA treated (0.1890 ±0.0056) g and (0.3862 ±0.0053) g than that of control group (0.2620 ±0.0020) g and (0.4867 ±0.0052) g (P<0.01); the amount of hepatic lipid droplet was fewer in VA treated than that of control group; the expression levels of scavenger receptor-BⅠ(SR-BⅠ) and hormonesensitive lipase(HSL) mRNA were significantly higher in VA treated than that of control group(P<0.01); peroxisome proliferators activated receptor γ(PPARγ) and sterol regulatory element binding protein-1c (SREBP-1c) were significantly lower in VA treated than that of control group(P<0.05); triglyceride hydrolyase(TGH) mRNA was significantly higher in VA treated than that of control group(P<0.05); and the levels of serum CHO and HDL-C were significantly lower in VA treated than that of control group(P<0.01). Our results suggest that VA profits to fat degradation and cholesterol cleaning.
