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| Cloning and Subcellular Localization of a Transcription Factor Gene from Vitis pseudoreticulata |
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Abstract Abstract Full-length cDNA sequence of transcription factor VpRFL1 was cloned from Vitis pseudoreticulata W.T. Wang according to EST sequence and using RACE method. VpRFL1 genomic DNA sequence was cloned from V. pseudoreticulata W.T. Wang using PCR technique. Sequence analysis showed that the full-length of VpRFL1 (GenBank accession No. FJ356672) was 1324 bp, containing a 1053 bp open reading frame, which encoded 350 amino acid residues. The corresponding DNA sequence was 1599 bp, containing 3 exons and 2 introns. Nuclear localization signal of VpRFL1 was located at N-terminal 19~46 amino acid residues, and the 275~316 amino acid residues at C-end were rich in Cys, which was a C4C4 variant of RING finger. Transient expression of recombinant plasmid VpRFL1/ PBI221-GFP in onion(Allium cepa) epidermal cells showed that VpRFL1 was localized in cell nuclei. The results from the sequence feature and localization analysis suggest that VpRFL1 functions in nuclei and was supposed to be involve in biotic stresses pathway.
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Received: 05 March 2009
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Corresponding Authors:
Yue-Jin Wang
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