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| cDNA-AFLP differential expression analysis of genes related with aborting bud in Chinese cabbage |
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Abstract Abstract: cDNA-AFLP technology and bioinformatics analysis was used to analyze gene expression difference at 10 consecutive periods bud from bud aborted to normal bud in Chinese cabbage, 192 differentially expressed sequences were obtained, in which 88 sequences were chosen randomly to sequence, 72 novel expressed sequence tags (ESTs) were obtained after sequencing. By bioinformatics analyzing, the ESTs were divided into seven types, such as energy and metabolism, membrane and transport, transcript and translation, amino acid synthesizing and processing, signal conduction, and Unclear classified et al, moreover, 5 ESTs were newly found in Chinese cabbage bud aborting and had been entered to GenBank, their entering number were GD185906、GD185907、GD185908、GD185909 and GD185910 respectively. The acquirement of ESTs related with Chinese cabbage aborted bud may be helpful to elucidate the mechanism of Chinese cabbage aborted bud, and will provide important resource for finding novel gene.
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Received: 09 April 2009
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Corresponding Authors:
lu-gang zhang
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