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Abstract Abstract: In order to clone the full-length of gene related to egg laying performance and further study the molecular mechanisms for the process of egg laying in the Zi geese, the experiment was conducted to constructed the full-length cDNA library of the ovary in the Zi geese by SMART (Switching Mechanism at 5’ end of RNA Transcript) using λTriplEx2 as vector. The results showed that the titration of the cDNA library we constructed was 3.1×106 pfu/mL, the rate of recombination was 94 %, and the fragment length of inserted DNA ranged mainly from 0.5 to 2 kb. 120 clones were chosen at random for sequencing from the cDNA library, and after BLASTn analysis of cDNAs,there possible function were predict. The results show that cDNA library we constructed can provide a base for further study on the full length cloning of important genes of egg laying.
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Received: 11 March 2009
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