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| A new simple and rapid plant DNA extraction method for PCR analysis |
| 韦露450103198306212562李丽婷120103198310162928 |
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Abstract The new DNA extraction method we established was based on the traditional alkaline lysis method. We changed the volume of neutralization solution and introduced an isopropanol precipitated step. The result showed that DNA extracted by this method can amplify target genes fragments which were short than 600 bp. Only 1% expected material contained in other samples can generate the specific amplified. The whole process can be finished in 21 minutes, and the applicability of NaOH-based method is extended.
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Received: 14 April 2009
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Corresponding Authors:
韦露450103198306212562李丽婷120103198310162928
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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