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Abstract Single nucleotide polymorphisms (SNPs) of exons 2、3 of IGF-1 gene were detected in Bian chicken and two reference chicken populations (Jinghai yellow chicken and Youxi chicken). There were no polymorphisms detected in the amplified region of primer P1 in three chicken Breeds, which may indicate that the sequence of exon2 of IGF-1 gene were rather conserved. While the region amplified by primer P2 displayed polymorphisms. Three genotypes( AA、AB and BB) were all detected in three chicken breeds. The allele A was the predominance allele in Bian chicken and Jinghai yellow chicken and that was allele B in Youxi ckicken. Sequencing revealed two mutations (93A→G and 148G→A ) of IGF-1 gene in the genotype AA in comparison to the genotype BB. The former mutation did not cause any amino acid change, and the latter mutation resulted in an amino acid change of Glu→Lys. Compared with the sequence of IGF-1 of chicken in Genbank, we found the mutations were at the point 62bp and 117bp of Exon3 of IGF-1 respectively.
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Received: 20 February 2009
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