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| The combined Effects of bovine follicular fluid(BFF)and gonadotrophins(FSH and LH)on In Vitro Development of Bovine Preantral Follicles |
| 高 志花 |
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Abstract In the McCoy's 5a serum-free culture system, the combined effects of bovine follicular fluid(BFF) with gonadotrophins (FSH and LH) on in vitro growth, development, suvival and secretion of estradiol (E2) of preantral follicles during culture were studied. The results showed that adding individually different doses of BFF to the culture medium did not influence obviously on the development of preantral follicles, but it could promote the secretion of estradiol. Under the condition of addition with appropriate proportion of BFF in presence 10ng/ml LH and 50ng/ml FSH, the development and survival rates of bovine preantral follicles in each control groups were higher than the 0% BFF group. Especially for 10% BFF group, the development（78.52%）of preantral follicles after 6 days culture was higher strikingly than contrast group(49.28%) (p<0.05) and preantral follicles diameter increased to 33.7+4.8 μm by 12 days of culture, which was significantly higher than the other treatment group (p <0.01). In the meantime, antrum formation occurred in the 9th day of culture. Secretion of E2 for each BFF group became more higher in the presence of gonadotrophins(FSH and LH). The level of E2 in the 10% BFF group arrived the highest value(48.24pg/mL) in the sixth day of culture. All these reaveled that 10% BFF combined with a certain proportion of gonadotropin can stimulate strongly preantral follicles in vitro growth, development and survival and induced follicular cavity formation.
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Received: 27 March 2009
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Corresponding Authors:
高 志花
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