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Identification of Camellia oleifera Changlin series superior clones by SRAP molecular marker |
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Abstract Abstract: Cultivars identification is an important method to resolve seedling confused. SRAP were used to detect the genetic diversity of 12 Changlin series superior clones. 15 primers pairs were screened out and 423 SRAP sites were Amplified from these clones, of which 394 markers were polymorphic loci and 50 markers were unique fragments. There were 13 primers pairs which could identify all clones respectively. In order to improve the identification speed, 24 loci were selected to establish the DNA finger print of the 12 clones. Genetic distance and cluster analysis were done, and the results showed that there was a great genetic diversity among the clones. All of these provide the rationale for camellia oleifera breeding.
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Received: 24 August 2009
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