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| Isolation, Characterization and Expression Analysis of Novel Gene Vnp1 Related to Pear Scab Disease Resistance from Zaosu Pear |
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Abstract Plant resistance genes play an important role in genetic resistance mechanism of plants. Based on the disease resistance gene analogs Pb-Zs3 which was cloned from Zaosu pear in our previous study, a full-length cDNA sequence was acquired with the method of homology-based cloning, and this gene was named Vnp1(GenBank accession No. FJ763188). Bioinformatic Analysis revealed that Vnp1 was a novel gene, and its cDNA was 1200 base pairs. The complete open reading frame (ORF) is 921bp in length, encoding 306-residue protein with a calculated molecular mass of 34. 6 kD. Meanwhile, two domains which related to disease resistance NB-ARC and disease resistance protein were also detected in its encoding protein. What’s more, a sequence of apple EST project induced by Venturia inaequalis showed 71% identity with Vnp1 by homology comparison. Semi-Quantitative RT-PCR revealed that the transcript of Vnp1 was significantly enhanced in the leaves of pear under the inducing of Venturia nashicola. This demonstrated that Vnp1 was correlated with the resistance to pear scab.
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Received: 05 March 2009
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YANG Han, LI Yan, SHEN Chang-Wei, JIN Yu-Meng, SHI Xiao-Qian, XIE Chang-Yan, Mei Xin-Lan, XU Yang-Chun, DONG Cai-Xia. Cloning and Expression Analysis of Potassium Channel Gene PbAKT1 in Pear (Pyrus betulifolia)[J]. 农业生物技术学报, 2019, 27(8): 1341-1350. |
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