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Abstract Two DNA fragments encoding N-term of MSTN and INH respectively were incorporated into coding region of hepatitis B virus S gene at C-term and middle(between amino acid position 112 and 113) to construct a myostatin expression vector pVAX-SMI. The plasmid pVAX-SMI was identified by restriction endonuclease digestion and sequencing. pVAX-SMI was transfected into HeLa cells and the recombinant protein was detected by Western Blot. The result showed that the plasmid could express recombinant MSTN and INH with immunogenicity successfully. The pVAX-SMI expression vector was constructed successfully.
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Received: 30 June 2008
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