Abstract To illuminate the effect of nitric oxide (NO) on Sertoli Cell and provide the base for studying mechanism of spermatogenesis, Sertoli Cell of piglets were chose to study the effect of NO on microfilaments of Sertoli Cell. Sertoli Cells were treated with SNP. Cell viability, antioxygen enzymes activity and changes of p-38MAPKwere detected. The results showed as followed:low level NO could keep Sertoli Cells’ microtubule and cell viability normally,and high level NO could increase the activity of p-38MAPK of cells, decrease antioxygen ability and cell secretion and destroy the structure and distribution of microtubule. The results inferred that SNP resulted in the increase of NO of Sertoli cells, and decreased antioxygen ability. The high level of NO destroyed cell microtubule through activating p38MAPK.
