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| Pathogenic Studies on the Diseases of Avian Embryo in AA Broiler Breeder and the Detection of Its Main Pathogens by PCR |
| LIU Hong-qin;ZHU Rui-liang;SUN Qiu-yan;HU Xiao-na;BI Jian-min;LIU Hong-Zhen;LI Xin-cang, WANG Wei |
| College of Animal Science and Technology, Shandong Agricultural University, Taian 271018,China |
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Abstract A kind of disease appeared in one large-scale AA broiler breeder farm in Shandong Province with the symptom of embryo death, increase of weak chicken, ineffective immunity, stunted growth of 30 days chicken since June, 2004. Based on the epidemiology investigation and the pathogenic studies, it was showed that the co-infection of Bordetella avium, Salmonella, Escherichia coli, mycoplasma, Chicken infectious anemia virus(CIAV) and Viral arthritis virus(VAV) was the major cause of the disease. These pathogens spread vertically from the eggs to the embryos. The 16S rRNA gene of Bordetella avium, CIAV VP3 gene and VAV S1 gene were amplified by PCR and 783 582 and 532 bp specific bands were acquired respectively. The PCR products of CIAV VP3 gene and VAV S1 gene could hybridize with the corresponding probe and appear positive reaction.
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Received: 16 December 2005
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Corresponding Authors:
ZHU Rui-liang
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